The Use of Human Platelet Lysate as a Coating Substance for Adipose-Derived Stem Cell Expansion
Issued Date
2024-02-23
Resource Type
eISSN
27686698
Scopus ID
2-s2.0-85186742689
Pubmed ID
38420820
Journal Title
Frontiers in bioscience (Landmark edition)
Volume
29
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Frontiers in bioscience (Landmark edition) Vol.29 No.2 (2024) , 88
Suggested Citation
Tragoonlugkana P., Chitchongyingcharoen N., Pruksapong C., Hassan S., Ngamkham K., Kamprom W., Supokawej A. The Use of Human Platelet Lysate as a Coating Substance for Adipose-Derived Stem Cell Expansion. Frontiers in bioscience (Landmark edition) Vol.29 No.2 (2024) , 88. doi:10.31083/j.fbl2902088 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/97552
Title
The Use of Human Platelet Lysate as a Coating Substance for Adipose-Derived Stem Cell Expansion
Corresponding Author(s)
Other Contributor(s)
Abstract
BACKGROUND: Large-scale production of mesenchymal stromal cells is essential for sufficient therapeutic doses in regenerative medicine. However, long-term cultivation encounters limited cell growth and cellular aging. Therefore, an alternative cell culture approach that promotes proliferation and attenuates cell senescence is required. Human platelet lysate (HPL) is a potent supplement for in vitro cell expansion. Applying HPL as a coating material can potentially improve mesenchymal stromal cell cultures. METHOD: To examine the capacity of HPL, it was used to pre-coat a tissue culture plate for in vitro adipose-derived mesenchymal stromal cell expansion. Alterations in biological features of adipose-derived stem cells (ADSCs) were investigated, including cell adhesion assays, cell proliferation, population doubling time, and cellular senescence. RESULTS: ADSCs cultured on HPL-coated plates significantly increased cell adhesion rate, shortened population doubling time, and stimulated cell growth. The senescent cells were significantly decreased in ADSCs cultured in an HPL-coated plate, and the expression levels of senescence-associated genes, including p16, p21, and p53, were downregulated. Furthermore, Western blotting analysis revealed that HPL was enriched with fibronectin and vitronectin, essential cell adhesive proteins. CONCLUSIONS: HPL was effectively used as a coating material for ADSC expansions. Cellular cultivation on the HPL coating is an alternative approach for producing mesenchymal stromal cells.