Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1
2
Issued Date
2022-08-01
Resource Type
ISSN
24058440
Scopus ID
2-s2.0-85136508195
Journal Title
Heliyon
Volume
8
Issue
8
Rights Holder(s)
SCOPUS
Bibliographic Citation
Heliyon Vol.8 No.8 (2022)
Suggested Citation
Deetanya P., Sitthiyotha T., Chomanee N., Chunsrivirot S., Wangkanont K. Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1. Heliyon Vol.8 No.8 (2022). doi:10.1016/j.heliyon.2022.e10396 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86491
Title
Biochemical and ligand binding properties of recombinant Xenopus laevis cortical granule lectin-1
Author's Affiliation
Other Contributor(s)
Abstract
Intelectins are putative innate immune lectins that are found throughout chordates. The first intelectin reported was Xenopus laevis cortical granule lectin-1 (XCGL-1 or XL-35). XCGL-1 is critical in fertilization membrane development in Xenopus. Here, we explored the biochemical properties of XCGL-1. The cysteines responsible for forming intermolecular disulfide bonds were identified. XCGL-1 adopted a four-lobed structure as observed by electron microscopy. The full-length XCGL-1 and the carbohydrate recognition domain (CRD) bind galactose-containing carbohydrates at nanomolar to micromolar affinities. Molecular modeling suggested that galactoside ligands coordinated the binding site calcium ion and interacted with residues around the groove made available by the non-conserved substitution compared to human intelectin-1. Folding conditions for production of recombinant XCGL-1 CRD were also investigated. Our results not only provide new biochemical insights into the function of XCGL-1, but may also provide foundation for further applications of XCGL-1 as glycobiology tools.