Non-encapsulated trichinella spp., t. papuae, diminishes severity of dssinduced colitis in mice
1
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Poom Adisakwattana, ภูมิ อดิศักดิ์วัฒนา, Supaporn Nuamtanong, สุภาพร น่วมทะนง, Teera Kusolsuk, ธีระ กุศลสุข, Montri Chairoj, มนตรี ไชยโรจน์, Pa-Thai Yenchitsomanas, Urai Chaisri, อุไร ไชยศรี (2013). Non-encapsulated trichinella spp., t. papuae, diminishes severity of dssinduced colitis in mice. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63190
Title
Non-encapsulated trichinella spp., t. papuae, diminishes severity of dssinduced colitis in mice
Abstract
Background: Helminths use various mechanisms to avoid host immunity and protect themselves from
being eliminated. Despite evading host immune responses, immunosuppression and regulation
mechanisms elicit functions that diminish the adverse effects of unrelated inflammatory diseases.
Objective: We investigated whether helminthic infections can ameliorate inflammatory diseases.
Methods: Mice were infected with Trichinella papuae and then subjected to induced colitis through the
oral administration of dextran sulfate sodium (DSS). Macroscopic and microscopic examinations measured
weight loss, stool consistency, gross bleeding, colon length, and tissue inflammation. In addition, cytokine
expression was observed in colon tissue by SYBR real-time RT -PCR to investigate the Th1, Th2, and regulatory
cytokines.
Result: The results showed that T. papuae infection decreased the severity of DSS-induced colitis,
including weight loss, bloody diarrhea, shortening of colon, and colon tissue damage in mice (p < 0.05).
The expression level of IL-4 was high in the colons of DSS-treated mice without helminthic infection, while
infected mice with DSS treatment had lower IL-4 levels (p 0.05). Uninfected DSS-treated mice failed to
produce IL-10 mRNA in colon tissue, which may cause more severe colitis. In contrast, prior to T. papuae
infection DSS-treated mice had IL-10 levels in the colon significant lower than the normal and infected control
groups.
Conclusion: Our data provide the evidence that prior T. papuae infection can ameliorate DSS-induced
colitis in mice and may be considered for a novel therapeutic strategy against immunological diseases in the
future.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.133.