A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture
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item.page.dc.date.issued
2023-12-01
item.page.dc.type
item.page.dc.identifier.issn
0125877X
item.page.dc.identifier.eissn
22288694
item.page.dc.identifier.doi
item.page.dc.identifier.scopus
2-s2.0-85178668610
item.page.dc.identifier.pmid
33274953
item.page.oaire.citation.title
Asian Pacific Journal of Allergy and Immunology
item.page.oaire.citation.volume
41
item.page.oaire.citation.issue
4
item.page.oaire.citation.startPage
401
item.page.oaire.citation.endPage
406
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SCOPUS
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Asian Pacific Journal of Allergy and Immunology Vol.41 No.4 (2023) , 401-406
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Pluangnooch P., Phayangkhe C., Soontrapa K. A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture. Asian Pacific Journal of Allergy and Immunology Vol.41 No.4 (2023) , 401-406. 406. doi:10.12932/ap-140720-0913 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/91468
item.page.dc.title
A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture
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item.page.dc.description.abstract
Background: Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties. Objective: To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares. Methods: The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry. Results: The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules. Conclusion: Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.