A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture
Issued Date
2023-12-01
Resource Type
ISSN
0125877X
eISSN
22288694
Scopus ID
2-s2.0-85178668610
Pubmed ID
33274953
Journal Title
Asian Pacific Journal of Allergy and Immunology
Volume
41
Issue
4
Start Page
401
End Page
406
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology Vol.41 No.4 (2023) , 401-406
Suggested Citation
Pluangnooch P., Phayangkhe C., Soontrapa K. A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture. Asian Pacific Journal of Allergy and Immunology Vol.41 No.4 (2023) , 401-406. 406. doi:10.12932/ap-140720-0913 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/91468
Title
A comparison of cell-culture plasticwares for effective human cytokine-induced killer cell culture
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Background: Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties. Objective: To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares. Methods: The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry. Results: The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules. Conclusion: Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.