Construction of Heme-Binding Protein Deleted Strain and Using It as an Attenuated Vaccine Against Nocardia seriolae in Hybrid Snakehead (Channa maculata ♀ × Channa argus ♂)
Issued Date
2025-01-01
Resource Type
ISSN
01407775
eISSN
13652761
Scopus ID
2-s2.0-85218838539
Pubmed ID
39945098
Journal Title
Journal of Fish Diseases
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Fish Diseases (2025)
Suggested Citation
Wu Y., Liu Y., Yang H., Sirimanapong W., Huang T., Chen J., Xia L. Construction of Heme-Binding Protein Deleted Strain and Using It as an Attenuated Vaccine Against Nocardia seriolae in Hybrid Snakehead (Channa maculata ♀ × Channa argus ♂). Journal of Fish Diseases (2025). doi:10.1111/jfd.14090 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/105554
Title
Construction of Heme-Binding Protein Deleted Strain and Using It as an Attenuated Vaccine Against Nocardia seriolae in Hybrid Snakehead (Channa maculata ♀ × Channa argus ♂)
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Corresponding Author(s)
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Abstract
Nocardia seriolae is the main pathogen causing fish nocardiosis, which can infect various aquatic freshwater and marine fish species. We have previously confirmed that the heme-binding protein of N. seriolae (NsHBP) is a secreted protein relating to bacterial virulence. In the present study, a ΔNsHBP mutant of N. seriolae was successfully generated by deleting NsHBP gene, and it was further used for attenuated vaccine development and evaluation in hybrid snakehead (Channa maculata ♀ × Channa argus ♂). The LD50 of strain ΔNsHBP by intraperitoneal injection in hybrid snakehead was presented at 5.47 × 107 CFU/mL, significantly (p < 0.01) higher than that of the wild-type strain at 5.28 × 105 CFU/mL, indicating the virulent reduction of strain ΔNsHBP in comparison to wild-type strain. The live attenuated vaccine used strain ΔNsHBP at sub-clinical dosages (LD15, 1.26 × 107 CFU/mL) to immunise the fish by intraperitoneal injection for 56 days could provide a relative percentage survival (RPS) at 82.40% against artificial challenge with wild-type strain ZJ0503. Additionally, serum enzymes activities of lysozyme (LZM), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD) as well as specific antibody (IgM) titers were induced in fish following ΔNsHBP vaccination. Furthermore, the expression of immune-related genes (TNFα, IL-1β, MHCIα, MHCIIα, CD4 and CD8α) was significantly increased in comparison to the control group during immunisation period with ΔNsHBP in fish. Taken together, it is revealed that the deleted strain ΔNsHBP could be a potential candidate for live attenuated vaccine development to control fish nocardiosis in aquaculture.