Streamlining phage enumeration: A glass drop plate approach for lytic Pseudomonas bacteriophage titration
Issued Date
2025-12-01
Resource Type
eISSN
29501946
Scopus ID
2-s2.0-105018114288
Journal Title
Microbe Netherlands
Volume
9
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbe Netherlands Vol.9 (2025)
Suggested Citation
Daewang N., Angsujinda K., Chaikeeratisak V., Smith D.R., Assavalapsakul W., Supanwong K. Streamlining phage enumeration: A glass drop plate approach for lytic Pseudomonas bacteriophage titration. Microbe Netherlands Vol.9 (2025). doi:10.1016/j.microb.2025.100576 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/112596
Title
Streamlining phage enumeration: A glass drop plate approach for lytic Pseudomonas bacteriophage titration
Corresponding Author(s)
Other Contributor(s)
Abstract
Due to its reliability, the double-layer agar (DLA) method is widely used for quantifying bacteriophages; however, it is time-consuming, labor-intensive, and requires careful optimization. This study developed a glass drop plate (GDP) method for rapid phage titration, capable of delivering results within 3 h while allowing nine spots per plate for testing multiple dilutions and triplicates. The GDP method produced phage titers comparable to those obtained using the DLA technique, with consistent results across three phages: SPA01 (2.20 × 10<sup>10</sup> vs. 3.00 × 10<sup>10</sup> PFU/mL), SPA05 (1.50 × 10<sup>9</sup> vs. 1.93 × 10<sup>9</sup> PFU/mL), and JJ01 (2.60 × 10<sup>10</sup> vs. 7.67 × 10<sup>10</sup> PFU/mL). Notably, the method replaces disposable plastic tips with reusable glass pipettes, offering an eco-friendly and sustainable alternative. This simple, cost-effective approach reduces material consumption and supports high-throughput screening, making it well-suited for rapid phage quantification in environmental, industrial, and clinical applications.