Isoenzyme analyses of opisthorchis viverrini and fasciola giantica by isoelectric focusing in polyacrylamide gel
3
Issued Date
1990
Copyright Date
1990
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
vi, 93 leaves : ill. (some col.)
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1990
Suggested Citation
Nitaya Sritong Isoenzyme analyses of opisthorchis viverrini and fasciola giantica by isoelectric focusing in polyacrylamide gel. Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1990. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/103333
Title
Isoenzyme analyses of opisthorchis viverrini and fasciola giantica by isoelectric focusing in polyacrylamide gel
Alternative Title(s)
การศึกษาโครงสร้างของไอโซเอนไซม์ในพยาธิใบไม้ตับคนและสัตว์
Author(s)
Advisor(s)
Abstract
Opisthorchis viverrini and Fasciola gigantica are parasites residing in the liver of man and animals. The fluke, O. viverrini is common in fish-eating animals including man, whereas F. gigantica is found in herbivorous animals and occasionally in human. Isoenzymes of the adult's O. viverrini and F. gigantica were analyzed by isoelectric focusing in polyacrylamide gel at the same electrophoresis condition. The eleven enzymes studied were: acid phosphatase, alkaline phosphatase, esterase, hexokinase, lactate dehydrogenase, glutamate dehydrogenase, glucose-6-phosphate dehydrogenase, malate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase and 6-phosphogluconate dehydrogenase. Among those enzymes examined, acid phosphatase, phosphoglucoisomerase, phosphoglucomutase and malate dehydrogenase showed high enzyme activities, whereas alkaline phosphatase, esterase, lactate dehydrogenase, hexokinase and 6-phosphogluconate dehydrogenase showed low enzyme activities. All of the eleven enzymes were found in F. gigantica, but four of them, namely, alkaline phosphatase, esterase, glutamate dehydrogenase and lactate dehydrogenase were not detected in O. viverrini. Genotypic differences between the two species were clearly distinguished by three enzymatic systems, namely, acid phosphatase, phosphoglucoisomerase and phosphoglucomutase. Among the eleven enzymes, phosphoglucomutase isoenzyme variation was found in O. viverrini. This work indicates that O. viverrini and F. gigantica can be distinguished by their protein and isoenzyme patterns besides from their morphological differences.
Description
Environmental Biology (Mahidol University 1990)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Environmental Biology
Degree Grantor(s)
Mahidol University