Rapid Diagnosis of Plasmodium knowlesi Infection Utilizing the Loop-Mediated Isothermal Amplification Technique Coupled with a Lateral Flow Assay
Issued Date
2026-07-01
Resource Type
ISSN
25869981
eISSN
26300559
Scopus ID
2-s2.0-105038407786
Journal Title
Journal of Health Science and Medical Research
Volume
44
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Health Science and Medical Research Vol.44 No.4 (2026)
Suggested Citation
Khammane T., Sawangjaroen N., Srinoun K., Buncherd H., Tun A.W., Jitueakul S., Ratcha C., Imwong M., Thanapongpichat S. Rapid Diagnosis of Plasmodium knowlesi Infection Utilizing the Loop-Mediated Isothermal Amplification Technique Coupled with a Lateral Flow Assay. Journal of Health Science and Medical Research Vol.44 No.4 (2026). doi:10.31584/jhsmr.20261306 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116817
Title
Rapid Diagnosis of Plasmodium knowlesi Infection Utilizing the Loop-Mediated Isothermal Amplification Technique Coupled with a Lateral Flow Assay
Corresponding Author(s)
Other Contributor(s)
Abstract
Objective: To develop a highly sensitive and specific molecular diagnostic assay for detecting P. knowlesi by combining LAMP with lateral flow assay (LFA). Material and Methods: Six LAMP primers targeting the P. knowlesi 18S rRNA gene were designed, incorporating FITC and biotin labels into the FIP and LoopF primers. Specificity and sensitivity were evaluated using DNA from various Plasmodium species. Clinical samples were tested, and the results were visualized on LFA. Results: The PkLAMP-LFA assay demonstrated a sensitivity of 95.4% (95% CI: 84.5–99.4%) and a specificity of 100% (95% CI: 15.8–100%) compared to nested PCR. The limit of detection was 10 copies/µL. The assay produced results within 60 minutes and required only minimal equipment. Conclusion: The PkLAMP-LFA assay is a rapid, accurate, and field-deployable diagnostic tool for P. knowlesi, offering a practical solution for improved malaria detection and control in endemic regions.