CD47 and Calreticulin Expression in Breast Cancer Subtypes and Anti-CD47 Inhibitory Effects in Macrophage-mediated Phagocytosis
Issued Date
2024-11-01
Resource Type
eISSN
17917530
Scopus ID
2-s2.0-85208165925
Pubmed ID
39477308
Journal Title
Anticancer research
Volume
44
Issue
11
Start Page
4929
End Page
4940
Rights Holder(s)
SCOPUS
Bibliographic Citation
Anticancer research Vol.44 No.11 (2024) , 4929-4940
Suggested Citation
Chantaraamporn J., Pothipan P., Sakulterdkiat T., Khiankaew B., Lumkul L., Mutapat P., Phetchahwang P., Svasti J., Champattanachai V. CD47 and Calreticulin Expression in Breast Cancer Subtypes and Anti-CD47 Inhibitory Effects in Macrophage-mediated Phagocytosis. Anticancer research Vol.44 No.11 (2024) , 4929-4940. 4940. doi:10.21873/anticanres.17318 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101963
Title
CD47 and Calreticulin Expression in Breast Cancer Subtypes and Anti-CD47 Inhibitory Effects in Macrophage-mediated Phagocytosis
Corresponding Author(s)
Other Contributor(s)
Abstract
BACKGROUND/AIM: Macrophage-mediated cancer immune evasion is modulated by the balance between "the cluster of differentiation 47 (CD47), an anti-phagocytic signal" and "calreticulin (CALR), a pro-phagocytic signal". CD47 is highly expressed in various types of cancer. However, the expression profiles of CD47 and CALR in breast cancer, especially in different hormone receptor subtypes, and the effects of CD47 blockade in macrophage-mediated therapy are not well understood. MATERIALS AND METHODS: The expression levels of CD47 and CALR were investigated in breast cancer and adjacent normal tissues using immunohistochemistry. To study the effects of CD47 blockade therapy, CD47 and CALR expression in breast cancer cell lines were determined. In vitro macrophage-mediated phagocytosis of breast cancer upon treatment with a monoclonal CD47 antibody (B6H12) were performed. RESULTS: CD47 and CALR were overexpressed in breast cancer tissues and their up-regulation was associated with hormone receptor subtypes. Patients with Luminal A breast cancer had higher levels of CD47, while patients with triple-negative breast cancer (TNBC) had higher levels of CALR. The levels of CD47 and CALR were also elevated in breast cancer cell lines of Luminal A (MCF-7) and TNBC (MDA-MB-231) subtypes. Interestingly, the expression ratio of surface CD47/CALR was significantly higher in MCF-7. Moreover, in vitro phagocytosis assays revealed that blockage of CD47 enhanced macrophage-mediated activity in both cancer cells with dramatically higher degrees of phagocytosis in MCF-7. CONCLUSION: The expression profiles of CD47 and CALR in breast cancer subtypes and the benefit of CD47 blocking-based immunotherapy are herein provided.