Laboratory colonization of Aedes lineatopennis
8
Issued Date
2004
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Natee Puangmalee, Anuluck Junkum, Atchariya Jitpakdi, Benjawan Pitasawat, Narumon Komalamisra, นฤมล โกมลมิศร์, Pongsri Tippawangkosol, Kom Sukontason, Wej Choochote (2004). Laboratory colonization of Aedes lineatopennis. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63431
Title
Laboratory colonization of Aedes lineatopennis
Other Contributor(s)
Abstract
Aedes lineatopennis, a species member of subgenous Neomelaniconion, can be colonized for more than 10 successive generations from 30 egg batches of wild-caught femeles. The oviposited eggs need to be incubated in a moisture chamber at least 7 days for the complete embryonation, and following immersion of these eggs in the 0.25-2% hay-fermented water, egg hatching is stimulated of which 61-66% hatched. Larvae are easily reared in 0.25-1% hay-fermented water and provided with suspended powder of equal weight of wheat germ, dry yeast, and oatmeal as the food. Larval development was complete 4-6 days. The pupal stage lasted 3-4 days and nearly all pupae reached the adult stage (87-91%). The adults had to mate artificially and 5-day-old male was proven to be the best age for induced copulation. Three to five-day-old females kept in a paper cup were fed easily on anaesthetized golden hamster that was placed on the top-screen. The average number of egg per gravid female was 63.56 ± 22.93. Unfed females and males kept in a paper cup and fed on 5% multivitamin-syrup solution lived up to 43.17 ± 12.63 (9-69) and 15.90 ± 7.24 (2.39) days, respectively, in the insectarium condition of 27 ± 2°C and 70-80% R.H.
Description
Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.235.