Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood
Issued Date
2025-12-01
Resource Type
eISSN
20452322
Scopus ID
2-s2.0-105013209657
Pubmed ID
40796650
Journal Title
Scientific Reports
Volume
15
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports Vol.15 No.1 (2025)
Suggested Citation
Sutjarit N., Ruknarong L., Tanprasertkul C., Bhukhai K., Soe H.M.S.H., Kheolamai P., Manochantr S., Tantrawatpan C., Tantikanlayaporn D. Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood. Scientific Reports Vol.15 No.1 (2025). doi:10.1038/s41598-025-15647-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/111765
Title
Andrographolide promotes the ex vivo expansion of CD34+ hematopoietic stem cells derived from human umbilical cord blood
Corresponding Author(s)
Other Contributor(s)
Abstract
Umbilical cord blood (UCB) units are an alternative source of human hematopoietic stem cells (HSCs) for allogeneic stem cell transplants. A large quantity of HSCs is needed but the low number of accessible cells from UCB has been a significant limitation. Improving the ex vivo growth of HSCs while preserving their functioning is required. Here, we report that andrographolide (AP) enhanced the expansion of human UCB-derived HSCs (HSPCs) and pro-moted primitive HSCs (CD34<sup>+</sup>CD38<sup>−</sup>CD90<sup>+</sup>). AP also improved HSC functionality, evidenced by increased growth of colony-forming units and multilineage differentiation. AP upregulated genes involved in the Wnt/β-catenin and Notch signaling pathways. AP also modulated signaling pathways involved in HSC self-renewal, proliferation, survival, and differentiation, demonstrated by Nanostring analysis. The results of this study suggest that andrographolide enhances ex vivo UCB-HSC expansion while maintaining functionality and has potential for treatment of hematological diseases.