Generation of human induced pluripotent stem cell line (MUi034-A) from an unusual case of hydrops fetalis associated with homozygous hemoglobin Constant Spring
Issued Date
2022-12-01
Resource Type
ISSN
18735061
eISSN
18767753
Scopus ID
2-s2.0-85143644987
Pubmed ID
36427475
Journal Title
Stem Cell Research
Volume
65
Rights Holder(s)
SCOPUS
Bibliographic Citation
Stem Cell Research Vol.65 (2022)
Suggested Citation
Wongkummool W., Tong-ngam P., Munkongdee T., Tangprasittipap A., Paiboonsukwong K., Hongeng S., Fucharoen S., Charoenkwan P., Tubsuwan A. Generation of human induced pluripotent stem cell line (MUi034-A) from an unusual case of hydrops fetalis associated with homozygous hemoglobin Constant Spring. Stem Cell Research Vol.65 (2022). doi:10.1016/j.scr.2022.102979 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83509
Title
Generation of human induced pluripotent stem cell line (MUi034-A) from an unusual case of hydrops fetalis associated with homozygous hemoglobin Constant Spring
Other Contributor(s)
Abstract
Hemoglobin Constant Spring (HbCS) is unstable hemoglobin resulting from a nucleotide substitution at the termination codon of the HBA2 gene (c.427 T > C). The homozygous state for HbCS is non-transfusion dependent in adults. Nevertheless, severe anemia is often observed in fetuses. Here, human induced pluripotent stem cell line MUi034-A was generated from peripheral blood CD34+ hematopoietic stem/progenitor cells (HSPCs) derived from a 14-year-old female with homozygous HbCS who had a history of severe anemia and hydrops during fetal period. The MUi034-A cell line represented embryonic-like characteristics as they expressed specific pluripotency markers, differentiated into the three germ layers, and retained normal karyotyping.