Cellular immune response to opisthorchis viverrine : in vitro study of mitogenic effect of mtaceercarizl and adult antigens
3
Issued Date
1992
Copyright Date
1992
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 68 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Pathobiology))--Mahidol University, 1992
Suggested Citation
Asatara Kantasewi Cellular immune response to opisthorchis viverrine : in vitro study of mitogenic effect of mtaceercarizl and adult antigens. Thesis (M.Sc. (Pathobiology))--Mahidol University, 1992. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/103200
Title
Cellular immune response to opisthorchis viverrine : in vitro study of mitogenic effect of mtaceercarizl and adult antigens
Alternative Title(s)
การศึกษาผลของตัวอ่อนและตัวเต็มวัยของพยาธิใบไม้ในตับต่อการกระตุ้นระบบภูมิคุ้มกันด้านเซลล์
Author(s)
Abstract
Opisthorchiasis, a common disease which is a public health problem~of Thai people especially in the Northeastern part of Thailand. Chronic infection in patients of these parasitic disease mostly occurred with bile duct cancer, cholangiocarcinoma and probably hepatocellular carcinoma. Moreover, patients who had recovered from the disease were able to have re-infection again. Cellular immune response was studied by cellular proliferation assay and IL-2 detection in patients and animal model which infected with Opisthorchis viverrini (O.V.). Blood samples taken from ten patients with egg positive in their stool from endemic area, were isolated for mononuclear cell (PBMC) population and activated with metacercarial and adult O.V. antigens compared with nonspecific mitogens; Phytohaemagglutinin (PHA), Concanavalin A (Con A). Whereas forty 0. viverrini infected hamsters were used as animal model. Splenic cells were prepared and activated to all above mentioned antigens in comparison with those of uninfected control hamsters. The results showed that both stages of 0. viverr in i antigens exhibit their mitogenic activity, since they are able to stimulate normal human PBMC and normal hamsters splenic cells. Normal human PBMC had relative higher proliferative response to metacercarial antigen than adult antigens. While in normal hamsters, no difference in stimulation the response between metacercarial and O.V. adult antigens were observed. The mitogenic activity are found in the early period as well as mitogens stimulation. The cells in the disease stage of patients and infected hamsters were able to respond to PHA and Con A stimulation, which delineated their usual activity. Infected hamster showed higher response to both stages of antigen at two different periods of infection, 3 and 48 weeks of experimental period. Whereas in patients, response to adult antigen stimution were found and high concentration 5 ug/ml of metacercarial antigen. IL-2 was able to detect in patients as well as in normal individuals. which represented their normal production function. Infected hamsters at 48 weeks of experimental period, production-of IL-2 were not correlated with the increasing of cellular response. This lymphoproliferative disorder might be due to the impairment of the immunoregulatory system in chronic stage of disease or probably correlate with the carcinogenesis mechanism.
Description
Pathobiology (Mahidol University 1992)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Pathobiology
Degree Grantor(s)
Mahidol University