Molecular properties and ligand specificity of zebrafish intelectin-2
2
Issued Date
2022-04-01
Resource Type
ISSN
10504648
eISSN
10959947
Scopus ID
2-s2.0-85127317169
Pubmed ID
35337980
Journal Title
Fish and Shellfish Immunology
Volume
123
Start Page
528
End Page
536
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology Vol.123 (2022) , 528-536
Suggested Citation
Singrang N., Sitthiyotha T., Chomanee N., Watthanasak C., Chunsrivirot S., Wangkanont K. Molecular properties and ligand specificity of zebrafish intelectin-2. Fish and Shellfish Immunology Vol.123 (2022) , 528-536. 536. doi:10.1016/j.fsi.2022.03.025 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83286
Title
Molecular properties and ligand specificity of zebrafish intelectin-2
Author's Affiliation
Other Contributor(s)
Abstract
Intelectins are immune lectins expressed in chordates, including several fish species, in which intelectins are known to be upregulated upon infection. However, the basic biochemical properties and bacteria binding specificities of several fish intelectins are not well studied. We focus our investigation on zebrafish intelectin-2 (DrIntL-2) that is predominantly expressed in the gastrointestinal tract. The disulfide-linked oligomeric state and the cysteine responsible for intermolecular disulfide bonds are identified. DrIntL-2 is a globular particle of around 30 nm. In addition to the typical exocyclic 1,2-diol ligands, DrIntL-2 binds β-1,3-glucan and recognizes Salmonella typhimurium and Pseudomonas aeruginosa. This investigation not only shed light on the fish innate immunity that will be essential for the aquaculture industry, but will also provide a foundation for further application of DrIntL-2 in bacteria detection and identification.