Prx4 acts as DAMP in shrimp, enhancing bacterial resistance via the toll pathway and prophenoloxidase activation
Issued Date
2023-01-20
Resource Type
eISSN
25890042
Scopus ID
2-s2.0-85144860902
Journal Title
iScience
Volume
26
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
iScience Vol.26 No.1 (2023)
Suggested Citation
Wanvimonsuk S., Jaree P., Kawai T., Somboonwiwat K. Prx4 acts as DAMP in shrimp, enhancing bacterial resistance via the toll pathway and prophenoloxidase activation. iScience Vol.26 No.1 (2023). doi:10.1016/j.isci.2022.105793 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82158
Title
Prx4 acts as DAMP in shrimp, enhancing bacterial resistance via the toll pathway and prophenoloxidase activation
Author(s)
Other Contributor(s)
Abstract
Peroxiredoxin (Prx), an antioxidant enzyme family, has been identified as immune modulating damage-associated molecular patterns (DAMPs) in mammals but not in shrimp. Acute non-lethal heat shock (NLHS) that enhances shrimp Penaeus vannamei resistance to Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (VPAHPND). Among the five P. vannamei Prxs (LvPrx) isoforms, LvPrx4, the most abundant in unchallenged shrimp hemocytes that was upregulated in hemocytes following NLHS treatment, is of great interest. The escalation of the LvPrx4 monomer in hemolymph of NLHS treated shrimp indicates that it probably acts as DAMP. This study revealed that pre-challenge with rLvPrx4 could prolong VPAHPND-infected shrimp survival, increase prophenoloxidase (proPO) activity and promote Toll pathway-related genes expression mediated by Toll-like receptor (TLR) 1 and 2. The presented findings elucidated the molecular mechanism of LvPrx4 monomer as DAMP in NLHS-induced VPAHPND resistance by inducing the TLR1/2 signaling pathway and the proPO activating system.
