Ultrastructural characterization of host-parasite interactions of Plasmodium coatneyi in rhesus macaques
1
Issued Date
2022-02-04
Resource Type
ISSN
00311820
eISSN
14698161
Scopus ID
2-s2.0-85116938149
Pubmed ID
35234595
Journal Title
Parasitology
Volume
149
Issue
2
Start Page
161
End Page
170
Rights Holder(s)
SCOPUS
Bibliographic Citation
Parasitology Vol.149 No.2 (2022) , 161-170
Suggested Citation
Lombardini E.D., Malleret B., Rungojn A., Popruk N., Kaewamatawong T., Brown A.E., Turner G.D.H., Russell B., Ferguson D.J.P. Ultrastructural characterization of host-parasite interactions of Plasmodium coatneyi in rhesus macaques. Parasitology Vol.149 No.2 (2022) , 161-170. 170. doi:10.1017/S0031182021001669 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83324
Title
Ultrastructural characterization of host-parasite interactions of Plasmodium coatneyi in rhesus macaques
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
A-Star, Singapore Immunology Network
Chulalongkorn University
NUS Yong Loo Lin School of Medicine
University of Otago
Armed Forces Research Institute of Medical Sciences, Thailand
Oxford Brookes University
Mahidol University
Nuffield Department of Medicine
University of Oxford Medical Sciences Division
A-Star, Singapore Immunology Network
Chulalongkorn University
NUS Yong Loo Lin School of Medicine
University of Otago
Armed Forces Research Institute of Medical Sciences, Thailand
Oxford Brookes University
Mahidol University
Nuffield Department of Medicine
University of Oxford Medical Sciences Division
Other Contributor(s)
Abstract
Plasmodium coatneyi has been proposed as an animal model for human Plasmodium falciparum malaria as it appears to replicate many aspects of pathogenesis and clinical symptomology. As part of the ongoing evaluation of the rhesus macaque model of severe malaria, a detailed ultrastructural analysis of the interaction between the parasite and both the host erythrocytes and the microvasculature was undertaken. Tissue (brain, heart and kidney) from splenectomized rhesus macaques and blood from spleen-intact animals infected with P. coatneyi were examined by electron microscopy. In all three tissues, similar interactions (sequestration) between infected red blood cells (iRBC) and blood vessels were observed with evidence of rosette and auto-agglutinate formation. The iRBCs possessed caveolae similar to P. vivax and knob-like structures similar to P. falciparum. However, the knobs often appeared incompletely formed in the splenectomized animals in contrast to the intact knobs exhibited by spleen intact animals. Plasmodium coatneyi infection in the monkey replicates many of the ultrastructural features particularly associated with P. falciparum in humans and as such supports its use as a suitable animal model. However, the possible effect on host-parasite interactions and the pathogenesis of disease due to the use of splenectomized animals needs to be taken into consideration.