Mimotope identification of human neutralizing monoclonal antibodies against dengue virus using random peptide phage display library
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Nataya Muenngern, นาตยา เหมือนเงิน (2013). Mimotope identification of human neutralizing monoclonal antibodies against dengue virus using random peptide phage display library. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63360
Title
Mimotope identification of human neutralizing monoclonal antibodies against dengue virus using random peptide phage display library
Author(s)
Abstract
Background: Dengue virus is the fastest emerging arboviral infection spread by Aedes mosquitoes with
major public health consequences in over 100 tropical and sub-tropical regions. The DENV complex
consists of four distinct but related viruses, designated as serotypes. Currently, there is no specific
antiviral or vaccine is available for dengue virus infection. However, many researchers have been trying
to develop the therapeutic agents against DENV. Phage display technology is a powerful method recently
developed to screen selective repertories from large diversity of combinatorial peptide libraries displayed on
the surface of bacteriophage. Methods: Two Human monoclonal antibodies (huMAbs) that show cross-neutralizing activity to
4 serotypes of DENV were produced from previous study (Setthapramote et al., 2012) and used as target
antibodies for epitope study. Random 7 and 12 position peptide libraries were used for selection by 3 rounds
of panning, and checked the target-specific phage clones by ELISA, before sending the M13 phage plasmids
for sequencing. Results: The MAbs 19 and 54 recognized phages displaying peptides with the consensus motif
LXXXG and LXGXG, respectively, that matched to conserved region of dengue E protein at position (107)
LFGKG(111). However, for further specificity confirmation of the peptide, it need to be synthesized and
checked the specificity by ELISA. The results described in this study will open avenues of development for
dengue diagnostic assays and the technique can be applied for other antibodies specific to other tropical
diseases.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.165.