Association of granulysin, perforin, ifn-γ and lymphocyte subsets influences the clinical outcome in thai patients with tb and hiv/tb coinfection
6
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Nada Pitabut, นฎา ปิตะบุตร, Shinsaku Sakurada, Takahiro Tanaka, Panadda Dhepakson, Hideki Yanai, Norio Yamada, Masaji Okada, Naoto Keicho, Srisin Khusmith, ศรีสิน คูสมิทธิ์ (2013). Association of granulysin, perforin, ifn-γ and lymphocyte subsets influences the clinical outcome in thai patients with tb and hiv/tb coinfection. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/63358
Title
Association of granulysin, perforin, ifn-γ and lymphocyte subsets influences the clinical outcome in thai patients with tb and hiv/tb coinfection
Abstract
Host effector mechanism against Mycobacterium tuberculosis infection is dependent on innate immune
response by macrophages and neutrophils and alterations in balanced adaptive immunity.
Coordinated release of cytolytic effector molecules from NK cells and effector T cells and the
subsequent granule-associated killing of infected cells have been documented; however, their potential role in
clinical tuberculosis is still controversy. This study aims to investigate whether circulating granulysin and other
effector molecules are associated with the number of NK cells, iNKT cells, Vγ9+Vδ2+ T cells, CD4+ T cells and
CD8+ T cells, and such association influences the clinical outcome of the disease in patients with pulmonary
TB and HIV/TB coinfection who have been living in Chiang Rai, north of Thailand where TB is endemic.
Granulysin and perforin levels in TB patients were lower than healthy controls, whereas much higher
granulysin levels in HIV/TB coinfection than in any other groups, TB and HIV with or without receiving
HAART were noted. Such higher levels corresponded to the number of CD8+ T cells which kept high, but
not with NK cells and other possible cellular sources of granulysin. In addition, the 17kDa, 15kDa and 9kDa
isoforms of granulysin were recognized in plasma of HIV/TB coinfection. Increased granulysin and decreased
IFN-γ levels in HIV/TB coinfection and TB after completion of anti-TB therapy were observed. The results
suggested that the alteration of circulating granulysin has potential function in host immune response against
TB and HIV/TB coinfection. This is the first demonstration so far of granulysin in HIV/TB coinfection.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.162.