Nationwide surveillance in Thailand revealed genotype-dependent dissemination of carbapenem-resistant Enterobacterales
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2022-01-01
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20575858
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2-s2.0-85128560894
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35438076
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Microbial Genomics
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8
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4
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SCOPUS
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Microbial Genomics Vol.8 No.4 (2022)
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Takeuchi D., Kerdsin A., Akeda Y., Sugawara Y., Sakamoto N., Matsumoto Y., Motooka D., Ishihara T., Nishi I., Laolerd W., Santanirand P., Yamamoto N., Tomono K., Hamada S. Nationwide surveillance in Thailand revealed genotype-dependent dissemination of carbapenem-resistant Enterobacterales. Microbial Genomics Vol.8 No.4 (2022). doi:10.1099/mgen.0.000797 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83915
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Nationwide surveillance in Thailand revealed genotype-dependent dissemination of carbapenem-resistant Enterobacterales
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Carbapenem-resistant Enterobacterales (CRE) are a serious public health threat because of their rapid dissemination. To determine the epidemiological and genetic characteristics of CRE infections in Thailand, we performed whole-genome sequencing of 577 carbapenem-resistant Klebsiella pneumoniae isolates and 170 carbapenem-resistant Escherichia coli isolates from hospitals across the nation. The four most prevalent carbapenemase genes harboured by these bacteria were blaNDM-1, blaNDM-5, blaOXA-181 and blaOXA-232. The gene blaNDM-1 was identified in diverse sequence types. The gene blaNDM-5 was identified almost exclu-sively in E. coli. The genes blaOXA-181, blaOXA-232, and co-carriage of blaNDM-1 and blaOXA-232 were found in specific sequence types from certain provinces. Replicon typing revealed the diverse backbones of blaNDM-1-and blaNDM-5-harbouring plasmids and successful expansion of blaNDM-1-harbouring IncN2-type plasmids. Core-genome single-nucleotide polymorphism analysis suggested that blaOXA-181-, blaOXA-232-, blaNDM-5-, and co-carriage of blaNDM-1 and blaOXA-232-associated sub-clonal lineages have recently predomi-nated in the provinces from where these isolates were isolated. Thus, we demonstrate genotype-dependent dissemination of CRE in Thailand, which is helpful for establishing infection-control strategies in CRE-endemic areas.