The Expression of miR-30b-5p during Erythropoiesis

Abstract

Background: Erythropoiesis, a process in which hematopoietic stem cells differentiate into mature red blood cells, is controlled by multiple molecular pathways. GATA-1 is a major erythroid transcription factor, which regulates erythroid differentiation and anti-apoptosis. The cleavage of GATA-1 by caspase-3 results in erythroid maturation arrested and apoptosis. To prevent the cleavage of GATA-1, the chaperone Heat Shock Protein 70 (Hsp70) translocates to the nucleus, enabling continued terminal maturation. The accumulation of Hsp70 in the nucleus is regulated by exportin-1 (XPO1), a nuclear export receptor, responsible for exporting Hsp70. Thus, targeting the Hsp70 nuclear translocation by blocking XPO1 could be a novel therapeutic option for dyserythropoiesis. microRNAs (miRNAs), small non-coding RNAs, regulate gene expression at the post-transcriptional level. Therefore, identifying miRNAs responsible for regulating XPO1 could offer a novel therapeutic approach. Method: The bioinformatic-based approaches (miRDB, miRmap, and miRTarBase) were utilized to identify potential miRNAs targeting XPO1. Among these miRNAs, miR-30b-5p was identified as a candidate for regulating XPO1. Subsequently, the expression pattern of miR-30b-5p was determined by RT-qPCR. Result: The results showed that the trend of miR-30b-5p expression was high on day 7 in which the main population was polychromatic erythroblast and markedly decreased on day 11, when orthochromatic erythroblasts were the main population. Conclusion: The differential expression of miR-30b-5p during erythropoiesis suggested that miR-30b-5p might play a role in the regulation of XPO1 expression. Regulation of miR-30b-5p expression could be a potential target for a novel therapeutic option for dyserythropoiesis. However, further study of XPO1 expression modulation by miR-30b-5p in dyserythropoiesis is required.