Gut microbiome in advanced non-small cell lung cancer: effect of chemotherapy and impact on efficacy
Issued Date
2026-05-31
Resource Type
ISSN
22186751
eISSN
22264477
Scopus ID
2-s2.0-105040548731
Journal Title
Translational Lung Cancer Research
Volume
15
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Translational Lung Cancer Research Vol.15 No.5 (2026)
Suggested Citation
Trachu N., Sensorn I., Khiewngam K., Monnamo N., Chantratita W., Sirachainan E., Reungwetwattana T., Oranratnachai S. Gut microbiome in advanced non-small cell lung cancer: effect of chemotherapy and impact on efficacy. Translational Lung Cancer Research Vol.15 No.5 (2026). doi:10.21037/tlcr-2026-1-0209 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/117137
Title
Gut microbiome in advanced non-small cell lung cancer: effect of chemotherapy and impact on efficacy
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: While evidence linking the gut microbiome (GM) to cancer immunotherapy is growing, data regarding its role in chemotherapy remains limited. This study aims to investigate the effect of chemotherapy on GM composition and its potential as a predictive biomarker for treatment outcomes in advanced non-small cell lung cancer (NSCLC). Methods: Advanced NSCLC patients treated with chemotherapy at Ramathibodi Hospital were prospectively enrolled. Clinical data and stool samples were collected at three time points: baseline, post-evaluation, and at progression of disease (PD). Fecal bacterial DNA was extracted, followed by PacBio Sequel II sequencing and comprehensive bioinformatic analysis. Clinical data were summarized using descriptive statistics. Progression-free survival (PFS) and overall survival (OS) were estimated by the Kaplan-Meier method, and predictive factors were identified using Cox-regression analysis. Results: This study analyzed 54 stool samples from 27 NSCLC patients treated with platinum-doublet chemotherapy. The median PFS and OS were 5.3 months [95% confidence interval (CI): 2.4-8.4] and 13.8 months (95% CI: 5.2-not reached), respectively. Post-chemotherapy changes (n=20 paired samples) showed a significant decrease in microbial richness, as evidenced by reduced Abundance-based Coverage Estimator (ACE) (P=0.02) and Chao1 (P=0.03) alpha diversity indices. Taxonomically, the relative abundance of Enterobacter was significantly decreased post-chemotherapy (P=0.03). Regarding treatment response (n=26 evaluable patients; 13 PD, 13 clinical benefit), baseline alpha diversity was not predictive of outcome. However, the relative abundance of Akkermansia was notably higher in the clinical benefit group, approaching statistical significance (P=0.07). Conclusions: Chemotherapy significantly reduced GM by decreasing species richness (as measured by the ACE and Chao1 index), while species diversity (as measured by the Shannon and Simpson index) remained unchanged. Therefore, confirming the definitive role of the GM as a predictive biomarker in chemotherapy-treated NSCLC patients necessitates further investigation in a larger, more robustly powered cohort.