Validation of UHPLC-ESI-MS/MS Method for Determining Steviol Glycoside and Its Derivatives in Foods and Beverages
Issued Date
2023-11-01
Resource Type
eISSN
23048158
Scopus ID
2-s2.0-85176544699
Journal Title
Foods
Volume
12
Issue
21
Rights Holder(s)
SCOPUS
Bibliographic Citation
Foods Vol.12 No.21 (2023)
Suggested Citation
Phungsiangdee Y., Chaothong P., Karnpanit W., Tanaviyutpakdee P. Validation of UHPLC-ESI-MS/MS Method for Determining Steviol Glycoside and Its Derivatives in Foods and Beverages. Foods Vol.12 No.21 (2023). doi:10.3390/foods12213941 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/91130
Title
Validation of UHPLC-ESI-MS/MS Method for Determining Steviol Glycoside and Its Derivatives in Foods and Beverages
Author's Affiliation
Other Contributor(s)
Abstract
The aim of this study was to validate a method for determining nine types of steviol glycoside and its derivatives in food and beverage products, using ultrahigh-performance liquid chromatography tandem mass spectrometry with electrospray ionization (UHPLC ESI MS/MS). The performance characteristics of the analysis method were determined along with their suitability for the intended use. Coefficient of determination (R2) calibration curves from 0.2 to 1.0 mg L−1 were in the ranges of 0.9911–0.9990, 0.9939–1.0000 and 0.9973–0.9999 for a beverage, yogurt and snack, respectively. Intra-day precisions in terms of percent relative standard deviation (% RSD) of concentration, at 0.2, 0.5 and 1.0 mg L−1, for the beverage, yogurt and snack were lower than 15% (1.1–9.3%). At all concentrations, percentage recoveries were in the accepted range of 70–120%. For the matrix effect study, matrix-matched calibration was used for all compounds, obtaining a linear concentration range from 0.2 mg L−1 to 1.0 mg L−1. Almost all matrix-matched results presented as percentage recoveries were within the accepted range of 80–120%. The limit of detection (LOD) for steviol glycosides ranged from 0.003 to 0.078 μg g−1, while the limit of quantitation (LOQ) ranged from 0.011 to 0.261 μg g−1. These results indicate that the modified test method can be applied to determine the presence of steviol glycoside and its derivatives in a wide range of sample matrices.