Eriodictyol Attenuates H<inf>2</inf>O<inf>2</inf>-Induced Oxidative Damage in Human Dermal Fibroblasts through Enhanced Capacity of Antioxidant Machinery
Issued Date
2022-06-01
Resource Type
eISSN
20726643
Scopus ID
2-s2.0-85132203407
Pubmed ID
35745283
Journal Title
Nutrients
Volume
14
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nutrients Vol.14 No.12 (2022)
Suggested Citation
Buranasudja V., Muangnoi C., Sanookpan K., Halim H., Sritularak B., Rojsitthisak P. Eriodictyol Attenuates H<inf>2</inf>O<inf>2</inf>-Induced Oxidative Damage in Human Dermal Fibroblasts through Enhanced Capacity of Antioxidant Machinery. Nutrients Vol.14 No.12 (2022). doi:10.3390/nu14122553 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83217
Title
Eriodictyol Attenuates H<inf>2</inf>O<inf>2</inf>-Induced Oxidative Damage in Human Dermal Fibroblasts through Enhanced Capacity of Antioxidant Machinery
Other Contributor(s)
Abstract
Oxidative stress in dermal fibroblasts is strongly correlated with the aging process of the skin. The application of natural compounds that can increase the ability of dermal fibroblasts to counteract oxidative stress is a promising approach to promote skin health and beauty. Eriodictyol is a flavonoid that exerts several pharmacological actions through its antioxidant properties. However, its protective effects on dermal fibroblasts have not yet been investigated. In this study, we investigated whether eriodictyol protects human dermal fibroblasts (BJ fibroblasts) from the harmful effects of hydrogen peroxide (H2O2). Eriodictyol pretreatment significantly prevented necrotic cell death caused by H2O2 exposure. In addition, the level of 2′,7′-dichloro-dihydro-fluorescein oxidation was decreased, and that of glutathione was maintained, indicating that the beneficial effects of eriodictyol against H2O2 were closely associated with oxidative-stress attenuation. Eriodictyol mediates its antioxidant effects on dermal fibroblasts against H2O2 through (i) the direct neutralization of reactive oxygen species; (ii) the enhancement of the activities of H2O2-detoxifying enzymes, including catalase and glutathione peroxidase; and (iii) the induction of the expressions of catalase and glutathione peroxidase 1 via the activation of the Nrf2 signaling system. These results support the potential application of eriodictyol as an ingredient in skincare products for cosmeceutical and pharmaceutical purposes.