The surface topography, composition and cell adhesion of commercially available laser-treated titanium implants
1
Issued Date
2024
Copyright Date
2019
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
vii, 38 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Dentistry))--Mahidol University, 2019
Suggested Citation
Kian, Khoo Lee, 1988 The surface topography, composition and cell adhesion of commercially available laser-treated titanium implants. Thesis (M.Sc. (Dentistry))--Mahidol University, 2019. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/92186
Title
The surface topography, composition and cell adhesion of commercially available laser-treated titanium implants
Author(s)
Abstract
Implant surface modification methods have recently involved laser treatments to achieve the desired implant surface characteristics. The objective of this research was to investigate the surface chemistry and topography of commercially available laser-irradiated Titanium implants, together with evaluating the cell morphology and cell adhesion of human fetal osteoblasts(hFOB) seeded onto the same implants. 6 commercially available laser-irradiated titanium implants were investigated. The surface topography of these implants was analyzed by scanning electron microscope (SEM) and the surface chemistry was evaluated with electron dispersive x-ray spectroscopy (EDS). The human fetal osteoblasts were seeded onto the implant fixtures to investigate the biocompatibility and adhesion. 2 out of 3 samples from Biomate® displayed dark areas under SEM. These dark areas were consistent with organic matter. In contrast, all Biohorizon® samples were free of dark areas. The hFOB cell experiments revealed cell adhesion with filipodia on Biomate® samples which was consistent with cell maturation. The cells on Biohorizon® samples were morphologically round and lacked projections; one sample was devoid of any noticeable cells under SEM. In conclusion, the presence of organic impurities in the Biomate® samples could not be overlooked because in an in vivo model, the disruption of the osseointegration process may occur due to the rejection of the biomaterial. Nonetheless, there is currently insufficient evidence to link implant failure directly to carbon contaminated implant surfaces. Cell adhesion was observed early after 48 hours in laser-irradiated titanium from both companies. Further studies that determine the toxicity of Vanadium from Ti6Al4V in an in-vivo environment and titanium alloys lasered to a similar roughness of Biomate® samples should indicate the reason for the disparity between the two implant brands.
Description
Dentistry (Mahidol University 2019)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Dentistry
Degree Discipline
Dentistry
Degree Grantor(s)
Mahidol University