Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile
7
item.page.dc.date.issued
2023-04-01
item.page.dc.type
item.page.dc.identifier.issn
01054538
item.page.dc.identifier.eissn
13989995
item.page.dc.identifier.doi
item.page.dc.identifier.scopus
2-s2.0-85139229211
item.page.dc.identifier.pmid
36153808
item.page.oaire.citation.title
Allergy: European Journal of Allergy and Clinical Immunology
item.page.oaire.citation.volume
78
item.page.oaire.citation.issue
4
item.page.oaire.citation.startPage
1088
item.page.oaire.citation.endPage
1103
item.page.dc.rights.holder
SCOPUS
item.page.dc.identifier.citation
Allergy: European Journal of Allergy and Clinical Immunology Vol.78 No.4 (2023) , 1088-1103
punsarn.item.page.citation
Wang L., Xiong Q., Saelim N., Wang L., Nong W., Wan A.T.Y., Shi M., Liu X., Cao Q., Hui J.H.L., Sookrung N., Leung T.F., Tungtrongchitr A., Tsui S.K.W. Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile. Allergy: European Journal of Allergy and Clinical Immunology Vol.78 No.4 (2023) , 1088-1103. 1103. doi:10.1111/all.15531 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81930
item.page.dc.title
Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile
item.page.dc.contributor.author
item.page.oairecerif.author.affiliation
item.page.dc.contributor.other
item.page.dc.description.abstract
Background: One of the most common cockroach types in urban areas, the American cockroach (Periplaneta americana), has been reported to impose an increased risk of allergies and asthma. Limited groups of allergens (Per a 1–13) have been identified in this species due to the lack of genome-related information. Methods: To expand the allergen profile of P. americana, genomic, transcriptomic, and proteomic approaches were applied. With the support of a high-quality genome assembled using nanopore, Illumina, and Hi-C sequencing techniques, potential allergens were identified based on protein homology. Then, using enzyme-linked immunosorbent assay, selected allergens were tested in Thai patients allergic to P. americana. Results: A chromosomal-level genome of P. americana (3.06 Gb) has been assembled with 94.6% BUSCO completeness, and its contiguity has been significantly improved (N50 = 151 Mb). A comprehensive allergen profile has been characterized, with seven novel groups of allergens, including enolase (Per a 14), cytochrome C (Per a 15), cofilin (Per a 16), alpha-tubulin (Per a 17), cyclophilin (Per a 18), porin3 (Per a 19), and peroxiredoxin-6 (Per a 20), showing IgE sensitivity in enzyme-linked immunosorbent assay. A new isoallergen of tropomyosin (Per a 7.02) and multiple potential isoallergens of Per a 5 were revealed using bioinformatics and proteomic approaches. Additionally, comparative analysis of P. americana with the closely related Blattodea species revealed the possibility of cross-reaction. Conclusion: The high-quality genome and proteome of P. americana are beneficial in studying cockroach allergens at the molecular level. Seven novel allergen groups and one isoallergen in Per a 7 were identified.