Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile
Issued Date
2023-04-01
Resource Type
ISSN
01054538
eISSN
13989995
Scopus ID
2-s2.0-85139229211
Pubmed ID
36153808
Journal Title
Allergy: European Journal of Allergy and Clinical Immunology
Volume
78
Issue
4
Start Page
1088
End Page
1103
Rights Holder(s)
SCOPUS
Bibliographic Citation
Allergy: European Journal of Allergy and Clinical Immunology Vol.78 No.4 (2023) , 1088-1103
Suggested Citation
Wang L., Xiong Q., Saelim N., Wang L., Nong W., Wan A.T.Y., Shi M., Liu X., Cao Q., Hui J.H.L., Sookrung N., Leung T.F., Tungtrongchitr A., Tsui S.K.W. Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile. Allergy: European Journal of Allergy and Clinical Immunology Vol.78 No.4 (2023) , 1088-1103. 1103. doi:10.1111/all.15531 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81930
Title
Genome assembly and annotation of Periplaneta americana reveal a comprehensive cockroach allergen profile
Author's Affiliation
Other Contributor(s)
Abstract
Background: One of the most common cockroach types in urban areas, the American cockroach (Periplaneta americana), has been reported to impose an increased risk of allergies and asthma. Limited groups of allergens (Per a 1–13) have been identified in this species due to the lack of genome-related information. Methods: To expand the allergen profile of P. americana, genomic, transcriptomic, and proteomic approaches were applied. With the support of a high-quality genome assembled using nanopore, Illumina, and Hi-C sequencing techniques, potential allergens were identified based on protein homology. Then, using enzyme-linked immunosorbent assay, selected allergens were tested in Thai patients allergic to P. americana. Results: A chromosomal-level genome of P. americana (3.06 Gb) has been assembled with 94.6% BUSCO completeness, and its contiguity has been significantly improved (N50 = 151 Mb). A comprehensive allergen profile has been characterized, with seven novel groups of allergens, including enolase (Per a 14), cytochrome C (Per a 15), cofilin (Per a 16), alpha-tubulin (Per a 17), cyclophilin (Per a 18), porin3 (Per a 19), and peroxiredoxin-6 (Per a 20), showing IgE sensitivity in enzyme-linked immunosorbent assay. A new isoallergen of tropomyosin (Per a 7.02) and multiple potential isoallergens of Per a 5 were revealed using bioinformatics and proteomic approaches. Additionally, comparative analysis of P. americana with the closely related Blattodea species revealed the possibility of cross-reaction. Conclusion: The high-quality genome and proteome of P. americana are beneficial in studying cockroach allergens at the molecular level. Seven novel allergen groups and one isoallergen in Per a 7 were identified.