Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a
Issued Date
2025-01-01
Resource Type
ISSN
15353702
eISSN
15353699
Scopus ID
2-s2.0-105005291909
Journal Title
Experimental Biology and Medicine
Volume
250
Rights Holder(s)
SCOPUS
Bibliographic Citation
Experimental Biology and Medicine Vol.250 (2025)
Suggested Citation
Khamwut A., Nimnual J., Chomta N., Nimsamer P., Mayuramart O., Kaewsapsak P., Pasittungkul S., Poovorawan Y., Payungporn S. Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a. Experimental Biology and Medicine Vol.250 (2025). doi:10.3389/ebm.2025.10387 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/110375
Title
Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a
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Abstract
Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 102 and 103 copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.