Invariant Natural Killer T (iNKT) cells response in human melioidosis
Issued Date
2025-09-01
Resource Type
ISSN
0125877X
eISSN
22288694
Scopus ID
2-s2.0-105020819419
Pubmed ID
34953481
Journal Title
Asian Pacific Journal of Allergy and Immunology
Volume
43
Issue
3
Start Page
719
End Page
726
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology Vol.43 No.3 (2025) , 719-726
Suggested Citation
Kamuthachad L., Pisuttimarn P., Kasetthat T., Chetchotisakd P., Anunnatsiri S., Sermswan R.W., Watarai H., Matangkasombut P., Wongratanacheewin S. Invariant Natural Killer T (iNKT) cells response in human melioidosis. Asian Pacific Journal of Allergy and Immunology Vol.43 No.3 (2025) , 719-726. 726. doi:10.12932/ap-290821-1217 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/112999
Title
Invariant Natural Killer T (iNKT) cells response in human melioidosis
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: Melioidosis is an infectious disease caused by Burkholderia pseudomallei. In infected mice, IFN-γ can provide protection against B. pseudomallei infection. Invariant Natural Killer T (iNKT) cells are a subpopulation of T lymphocytes, activated by recognition of glycolipid ligands such as α-Galactosylceramide presented by CD1d, produce and secrete several cytokines, including IFN-γ and IL-4. The response of iNKT cells in human melioidosis was then investigated. Objectives: To determine the iNKT cells response in human melioidosis. Methods: The number of human iNKT cells and its activation states were investigated in sepsis melioidosis patients compared with healthy controls using flow cytometry. The iNKT cells activation was confirmed in vitro using heat-killed B. pseudomallei with normal peripheral blood mononuclear cells. The components induced iNKT cell were also determined using different concentration of B. pseudomallei lipopolysaccharide (LPS), heat-killed B. pseudomallei treated with or without DNase, RNase, or proteinase. Results: The number of human iNKT cells was significantly lower while the percentage of activated iNKT cells was higher in sepsis melioidosis when compared to control. In addition, B. pseudomallei can stimulate human iNKT cells in vitro. Heat-killed B. pseudomallei could activate iNKT cells but not relate to nucleic acid, proteins, or LPS. Conclusions: We found for the first time that the iNKT cells were activated during B. pseudomallei infection in human. However, the roles and the mechanism of iNKT cells during early state of infection needed to be further investigated.