Enhancement of surface sterilization protocol for in vitro propagation of Impatiens sirindhorniae
Issued Date
2022-02-01
Resource Type
ISSN
05677572
eISSN
24066168
Scopus ID
2-s2.0-85124210522
Journal Title
Acta Horticulturae
Volume
1334
Start Page
257
End Page
262
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Horticulturae Vol.1334 (2022) , 257-262
Suggested Citation
Samala S., Kongton K., Yenchon S., Petchsri S., Suwannakong Y., Rotjanajinda V., Buakaew P., Suleethat N., Thepsuriwong S., Pengjun W., Nongkaew S., Chareonsap P.P., Thammasiri K. Enhancement of surface sterilization protocol for in vitro propagation of Impatiens sirindhorniae. Acta Horticulturae Vol.1334 (2022) , 257-262. 262. doi:10.17660/ActaHortic.2022.1334.31 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83326
Title
Enhancement of surface sterilization protocol for in vitro propagation of Impatiens sirindhorniae
Other Contributor(s)
Abstract
A study on sterilization of leaf, nodal, and shoot explants of Impatiens sirindhorniae, an endemic species found only in Krabi and Suratthani provinces has been conducted. Plants were collected from the reservoir of Rajjaprabha Dam, Khao Sok National Park. The explants were cleaned by using detergent solution for 3-5 min and rinsed with tap water for 10-15 min. Then, the explants were immersed in different Clorox concentrations (10, 15, and 20%) or immersed in 0.1% mercuric chloride or immersed in 0.1% mercuric chloride in combination with 10, 15, and 20% Clorox. The explants were cultured on MS agar medium for 4 weeks at 25±2°C, under 16-h photoperiod with 60 photons µmol m-2 s-1 light intensity. The results showed that nodal explants that were sterilized using mercuric chloride at concentration of 0.1% for 20 min is the most effective technique. This treatment gave the survival rate of 83.3% and all the explants developed into shoots and roots. In contrary, survival leaf and shoot explants did not develop into shoots and roots.