The effect of 2% chlorhexidine iontophoresis on dentin sealing ability of etch-and-rinse adhesive: An in vitro study
Issued Date
2023-01-01
Resource Type
ISSN
19917902
eISSN
22138862
Scopus ID
2-s2.0-85170290481
Journal Title
Journal of Dental Sciences
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Dental Sciences (2023)
Suggested Citation
Kijsamanmith K., Srisatayasatien P., Thanindratarn N., Vichainarong C., Panyasukum J. The effect of 2% chlorhexidine iontophoresis on dentin sealing ability of etch-and-rinse adhesive: An in vitro study. Journal of Dental Sciences (2023). doi:10.1016/j.jds.2023.09.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/90168
Title
The effect of 2% chlorhexidine iontophoresis on dentin sealing ability of etch-and-rinse adhesive: An in vitro study
Author's Affiliation
Other Contributor(s)
Abstract
Background/purpose: Iontophoresis could enhance the delivery of chlorhexidine into oral tissue. This study aimed to determine the effect of 2% chlorhexidine iontophoresis (CHI) on the sealing ability of etch-and-rinse adhesive in human dentin using hydraulic conductance (HD) measurement, scanning electron microscopy and energy dispersive x-ray spectroscopy (SEM-EDS). Materials and methods: Thirty-nine sound dentin specimens were prepared from 39 extracted intact third molars. Thirty specimens were used for HD measurement and randomly divided into 3 equal-sized groups; (1) No chlorhexidine treatment (control), (2) passive chlorhexidine treatment (CHT) and (3) CHI on acid-etched dentin. Each dentin surface was treated with etch-and-rinse adhesive. HD of each specimen was measured before treatment, after immediate bonding and after 14 days. The other 9 specimens were subjected to SEM-EDS analysis of the acid-etched dentin and the dentin treated with CHT and CHI. ANOVA test and Student-Newman-Keuls method were used for statistical analysis. Results: After bonding, there was no significant difference in percentage decrease of HD among the treatment groups (P > 0.05). After 14 days, CHI and CHT groups had greater percentage decrease of HD than the control (P < 0.001 and P = 0.009, respectively). Under SEM-EDS analysis, acid-etched dentin with CHI presented opened dentinal tubule orifices and more chlorhexidine precipitates on dentin than the dentin with CHT, which strongly related to a higher percentage of chloride ions on the CHI dentin surface (P < 0.001). Conclusion: The use of CHI on acid-etched dentin had a positive effect on dentin sealing ability of etch-and-rinse adhesive.