Trypsin inhibiting effect and possible formation of nitroso compound of conventional Thai mushrooms
9
Issued Date
1992
Copyright Date
1992
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xi, 106 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1992
Suggested Citation
Naranin Markman Trypsin inhibiting effect and possible formation of nitroso compound of conventional Thai mushrooms. Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1992. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/103317
Title
Trypsin inhibiting effect and possible formation of nitroso compound of conventional Thai mushrooms
Alternative Title(s)
ผลการยับยั้งเอ็นไซม์ทริพซินและการเกิดสารประกอบไนโตรโซในเห็ดไทย
Author(s)
Advisor(s)
Abstract
The extracts of twenty one edible mushrooms namely, Agaricus bisporus (Lang) Sing (เห็ดกระดุม), Termitomyces fuliginosus (Heim) (เห็ดโคน), Amanita vaginata (Fr.) (เห็ดระโงก เหลือง), Agrocybe cylidacea Quel var fulva Gill (เห็ดยางาหงิ), Boieuts edulis (Bull), (เห็ดตับเต่า), Volvariella volvacea (เห็ดฟาง), Russula delica (Fr.) (เห็ดตะไคล), Clitocybe sp. (เห็ดขอนขาว), Lentinum edodes (เห็ดหอม), Pleurotus citrinopileatus (Vass.) Sing (เห็ดนางรมทอง), Pleurotus cornucopiae (Pers) Rolland (เห็ดนางรมขาว), Pleurotus cystidiosus (เห็ดเป๋าฮื้อ), Pleurotus eous (เห็ดนางรมภูฐาน), Pleurotus flabellatus (เห็ดนางนวล), Pleurotus sajor cauj (Fr.) sing (เห็ดนางฟ้า), Tricholoma crassum (Bert.) Sacc (เห็ดตีนแรด), Craterellus sp. (เห็ดขมิ้นน้อย), Ganoderma lucidum (เห็ดหลินจือ), Schizophyllum commune (เห็ดแคลง), Auricularia polytricha (เห็ดหูหนู หนาดอกน้ำตาล) and canned Astreaus sp. (เห็ดเผาะ) were studied on the apparent in vitro trypsin inhibitor (TI) activity, The determination on p-nitroanilline after hydrolysis of substrate, N- benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPA) with trypsin was performed. The results showed that only three mushroom species of Termitomyces fuliginosus (เห็ดโคน) Volvariella volvaces (เห็ดฟาง) and Tricholoma crassum (เห็ดตีนแรด) had antitryptic activity. However, TI presence in Volvariella voivacea (เห็ดฟาง) was 9.67 TUI/mg dry wt which provided higher activity than that of the other two mushrooms with very low activity compared with soybean trypsin inhibitor (SBTI). The effects of heat treatment on the destruction of TI in the aqueous extracts of mushroom were studied. TI of Volvariella volvacea (เห็ดฟาง) was more thermolabile than the inhibitors of soybean. The inhibitory activity remained 23.68 % after heated at 37(...)C for 24 hours. The TI of Volvariella volvacea (เห็ดฟาง), Termitomyces fuliginosus (เห็ดโคน), Tricholoma crassum (เห็ดตีนแรด) and soybean were almost completely destroyed after autoclaved at 121(...)C, 15 lb/in(...) for 30 minutes. Interestingly, destruction pattern of trypsin inhibitor activities of Termitomyces fuliginosus (เห็ดโคน) and Tricholoma crassum (เห็ดตีน แรด) were the same. All mushroom extracts produced considerable amount of N- nitroso morpholine (NMOR) after being nitrosated with exogeneous nitrite at pH 3.0-3.5 and 37C for 4 hours. The range were between 0.66 to 59.09 nmol/gram wet wt whereas the highest amount was found in the extract of Ganodermaa lucidum (เห็ดหลินจือ) and the lowest of Auricularia polytricha (เห็ดหูหนูหนาดอกน้ำตาล). The results from concomitant study demonstrated that some mushrooms had antitryptic activities. All species contained possible N-nitroso compound precursors after nitrosated in acidic condition. Therefore, the consumption of cooked mushroom and with an avoidance of nitrite-cured food might be good to health.
Description
Environmental Biology (Mahidol University 1992)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Environmental Biology
Degree Grantor(s)
Mahidol University