Ethyl Acetate Extract of Marine Algae, Halymenia durvillei, Provides Photoprotection against UV-Exposure in L929 and HaCaT Cells
Issued Date
2022-11-11
Resource Type
eISSN
16603397
Scopus ID
2-s2.0-85142627714
Pubmed ID
36421985
Journal Title
Marine drugs
Volume
20
Issue
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
Marine drugs Vol.20 No.11 (2022)
Suggested Citation
Kraokaew P., Manohong P., Prasertsuksri P., Jattujan P., Niamnont N., Tamtin M., Sobhon P., Meemon K. Ethyl Acetate Extract of Marine Algae, Halymenia durvillei, Provides Photoprotection against UV-Exposure in L929 and HaCaT Cells. Marine drugs Vol.20 No.11 (2022). doi:10.3390/md20110707 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86823
Title
Ethyl Acetate Extract of Marine Algae, Halymenia durvillei, Provides Photoprotection against UV-Exposure in L929 and HaCaT Cells
Other Contributor(s)
Abstract
Halymenia durvillei is a red alga distributed along the coasts of Southeast Asian countries including Thailand. Previous studies have shown that an ethyl acetate fraction of H. durvillei (HDEA), containing major compounds including n-hexadecanoic acid, 2-butyl-5-hexyloctahydro-1H-indene, 3-(hydroxyacetyl) indole and indole-3-carboxylic acid, possesses high antioxidant and anti-lung cancer activities. The present study demonstrated that HDEA could protect mouse skin fibroblasts (L929) and human immortalized keratinocytes (HaCaT) against photoaging due to ultraviolet A and B (UVA and UVB) by reducing intracellular reactive oxygen species (ROS) and expressions of matrix metalloproteinases (MMP1 and MMP3), as well as increasing Nrf2 nuclear translocation, upregulations of mRNA transcripts of antioxidant enzymes, including superoxide dismutase (SOD), heme oxygenase (HMOX) and glutathione S-transferase pi1 (GSTP1), and procollagen synthesis. The results indicate that HDEA has the potential to protect skin cells from UV irradiation through the activation of the Nrf2 pathway, which leads to decreasing intracellular ROS and MMP production, along with the restoration of skin collagen.