Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution
Issued Date
2022-02-01
Resource Type
ISSN
0001706X
eISSN
18736254
Scopus ID
2-s2.0-85121312870
Pubmed ID
34890540
Journal Title
Acta Tropica
Volume
226
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Tropica Vol.226 (2022)
Suggested Citation
Sangkanu S., Mitsuwan W., Mahboob T., Mahabusarakam W., Chewchanwuttiwong S., Siphakdi P., Jimoh T.O., Wilairatana P., Dolma K.G., Pereira M.d.L., Rahmatullah M., Wiart C., Norouzi R., Siyadatpanah A., Mutombo P.N., Nissapatorn V. Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution. Acta Tropica Vol.226 (2022). doi:10.1016/j.actatropica.2021.106266 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83335
Title
Phytochemical, anti-Acanthamoeba, and anti-adhesion properties of Garcinia mangostana flower as preventive contact lens solution
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
Islamic University in Uganda
Sikkim Manipal Institute of Medical Sciences
Chulalongkorn University
Walailak University
Birjand University of Medical Sciences
Universiti Malaysia Sabah
CICECO – Instituto de Materiais de Aveiro
Southern Cross University
University of Tabriz
Prince of Songkla University
University of Development Alternative
Islamic University in Uganda
Sikkim Manipal Institute of Medical Sciences
Chulalongkorn University
Walailak University
Birjand University of Medical Sciences
Universiti Malaysia Sabah
CICECO – Instituto de Materiais de Aveiro
Southern Cross University
University of Tabriz
Prince of Songkla University
University of Development Alternative
Other Contributor(s)
Abstract
Acanthamoeba keratitis infection extends due to the growing number of contact lens users. Indigenous plants including Garcinia mangostana play a vital role in human health and well being. Many species of this plant have been reported with myriads of potent medicinal properties. However, the aims of this study were, for the first time, to isolate compounds from the flower of G. mangostana and to test their anti-Acanthamoeba and anti-adhesion activity against Acanthamoeba triangularis. Powdered flowers of G. mangostana were extracted and chromatographed on a silica gel column. The structures of the compounds were established with the aid of 1H NMR. More so, the anti-Acanthamoeba and anti-adhesion properties were tested on a 96-well polystyrene microtiter plate and soft contact lenses. Scanning electron microscope (SEM) was used to determine the features of A. triangularis on contact lenses. Eight pure compounds were obtained, namely 9-hydroxycalabaxanthone, tovophillin A, garcinone E, garcinone B, α-mangostin, gartinin, 8-deoxygartinin and γ-mangostin. The extract and pure compounds exhibited anti-Acanthamoeba activity with MIC values in the range of 0.25–1 mg/mL. In addition, the extract and α-mangostin displayed significant activity against the adhesion of A. triangularis trophozoites both in polystyrene plate and in contact lenses at 0.5 × MIC (0.25 mg/mL). Furthermore, α-mangostin has the potential to remove A. triangularis adhesion in contact lenses similar to a commercial multipurpose solution (MPS). SEM study confirmed that crude extract and α-mangostin are effective as solutions for contact lenses, which removed A. triangularis trophozoites within 24 h. Alpha-mangostin was non-toxic to Vero cells at a concentration below 39 μM in 24 h. Crude extract of G. mangostana flower and its α-mangostin serve as candidate compounds in the treatment of Acanthamoeba infection or as lens care solution, since they can be used as a source of natural products against Acanthamoeba and virulence factor associated with the adhesion of A. triangularis.