Molecular Cloning and Characterization of a Fasciola gigantica Nuclear Receptor Subfamily 1 (FgNR1)
Issued Date
2022-12-01
Resource Type
eISSN
20760817
Scopus ID
2-s2.0-85144705257
Journal Title
Pathogens
Volume
11
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
Pathogens Vol.11 No.12 (2022)
Suggested Citation
Martviset P., Chantree P., Chaimon S., Torungkitmangmi N., Prathaphan P., Ruangtong J., Sornchuer P., Thongsepee N., Sangpairoj K., Adisakwattana P. Molecular Cloning and Characterization of a Fasciola gigantica Nuclear Receptor Subfamily 1 (FgNR1). Pathogens Vol.11 No.12 (2022). doi:10.3390/pathogens11121458 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83497
Title
Molecular Cloning and Characterization of a Fasciola gigantica Nuclear Receptor Subfamily 1 (FgNR1)
Other Contributor(s)
Abstract
Fasciola gigantica, a giant liver fluke, causes tremendous loss to the livestock economy in several regions throughout the world. The situation of drug resistance has been emerging increasingly; therefore, novel drugs and drug targets need to be discovered. The adult F. gigantica inhabits the major bile ducts where bile salts accumulate—these are steroid-like molecules that mediate several physiological processes in organisms through interacting with their specific nuclear receptors. However, the molecular mechanism of the interaction in the parasitic organisms have not been clearly understood. In this study, putative nuclear receptor subfamily 1 of F. gigantica (FgNR1) was identified. Nucleotide and amino acid sequences of the FgNR1 homolog were obtained from the transcriptome of F. gigantica and predicted for properties and functions using bioinformatics. The full-length cDNA was cloned and expressed in the bacterial expression system and then used for immunization. Western analysis and immunolocalization suggested that FgNR1 could be detected in the crude worm antigens and was highly expressed in the caeca and testes of the adult parasite. Moreover, the bile could significantly activate the expression of FgNR1 in cultured parasites. Our results indicated that FgNR1 has high potential for the development of a novel anthelminthic drug in the future.