Detection and identification of dengue viruses in suckling mice : comparison studies between the Western blot and viral gene amplification processes
Issued Date
1992
Copyright Date
1992
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
viii, 105 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Pathobiology))--Mahidol University, 1992
Suggested Citation
Chalenee Watanawanna Detection and identification of dengue viruses in suckling mice : comparison studies between the Western blot and viral gene amplification processes. Thesis (M.Sc. (Pathobiology))--Mahidol University, 1992. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/103212
Title
Detection and identification of dengue viruses in suckling mice : comparison studies between the Western blot and viral gene amplification processes
Alternative Title(s)
การตรวจสอบและพิสูจน์เชื้อไวรัสไข้เลือดออกในหนูทดลอง : การศึกษาเปรียบเทียบระหว่างการย้อมด้วยเอ็นไซม์บนแผ่นไนโตรเซลลูโลส และขบวนการเพิ่มจำนวนยีนไวรัส
Author(s)
Advisor(s)
Abstract
Dengue fever patients' sera, in the year 1990, were obtained from Ramathibodi Hospital. Detection and identification for the presence of dengue viruses that caused the hemorrhagic fever in children were done by passage of viruses to suckling mouses brain. This classical method was employed more than 40 years ago. The moribund were killed on day 6 or 7 and various infected organs were collected. The infected brain and liver were the first two organs that demonstrated the presence of dengue viruses study, investigation were extended to cover the spleen, lung, lymph nodes and plasma. The results from Viral Antigen Strip / Enzyme immunoassay (VAS/EIA) was compared to PCR technique. For PCR technique, both primers, sense and complementary, were synthesized and purified. Using the positive specimens screening by VAS/EIA as the starting material, the first PCR experiment was done by using suckling mice that were infected with dengue virus type 2 as a model. Since the specimens were from different type of tissue, several methods to extract viral RNA from these tissues were employed and the results were evaluated. The results indicated that the PCR method can detect viral genes in brain, liver, spleen, lymph nodes and which confirmed previous study using VAS/EIA. Improvement can be done via better extraction method which may lead to a sensitive system to detect dengue viral RNA in plasma and serum of infected mouse with an ultimate goal to identify the virus in clinically confirmed dengue fever cases.
Description
Pathobiology (Mahidol University 1992)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Pathobiology
Degree Grantor(s)
Mahidol University