Molecular characterization of human bocavirus in recycled water and sewage sludge in Thailand
Issued Date
2022-06-01
Resource Type
ISSN
15671348
eISSN
15677257
Scopus ID
2-s2.0-85127314037
Pubmed ID
35367361
Journal Title
Infection, Genetics and Evolution
Volume
100
Rights Holder(s)
SCOPUS
Bibliographic Citation
Infection, Genetics and Evolution Vol.100 (2022)
Suggested Citation
Booranathawornsom T., Pombubpa K., Tipayamongkholgul M., Kittigul L. Molecular characterization of human bocavirus in recycled water and sewage sludge in Thailand. Infection, Genetics and Evolution Vol.100 (2022). doi:10.1016/j.meegid.2022.105276 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83226
Title
Molecular characterization of human bocavirus in recycled water and sewage sludge in Thailand
Author's Affiliation
Other Contributor(s)
Abstract
The study aimed to assess the presence and molecular characterization of human bocavirus (HBoV) in recycled water and sewage sludge samples in Thailand. One hundred and two recycled water and eighty-six sewage sludge samples collected from a wastewater treatment plant were tested for the presence of HBoV using nested PCR with broad-range primer pairs targeting the capsid proteins VP1 and VP2. HBoV DNA was detected in recycled water of 9/102 (8.8%) samples and sewage sludge of 27/86 (31.4%) samples. Based on DNA sequencing and phylogenetic analysis, the HBoV DNA sequences had 98.8–100.0% nucleotide identity to the sequences from HBoV reported globally. Thirty-five HBoV-positive samples were identified to genotypes as the predominant HBoV2; 26 followed by HBoV3; 8 and the rare HBoV4; 1 sample. Concerning recycled water, HBoV2 was detected in 3 (2.9%) and HBoV3 was detected in 5 (4.9%) of all samples. The sewage sludge samples were characterized as HBoV2 in 23 (26.7%), HBoV3 in 3 (3.5%) and HBoV4 in 1 (1.2%) of all samples. The frequency of HBoV detected in recycled water and sewage sludge samples significantly differed in sample type (p-value = 0.007). The findings of three HBoV genotypes in recycled water and sewage sludge emphasized the circulation of the virus in the environment and the potential source of transmission to the community.