Evaluation Of Plasma Cardiac Troponin I In Asian Elephants (Elephas Maximus) Using Two Clinical Analyzers
Issued Date
2023-01-05
Resource Type
ISSN
10427260
Scopus ID
2-s2.0-85146364727
Pubmed ID
36640066
Journal Title
Journal of Zoo and Wildlife Medicine
Volume
53
Issue
4
Start Page
654
End Page
660
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Zoo and Wildlife Medicine Vol.53 No.4 (2023) , 654-660
Suggested Citation
Gallini S.H., Digeronimo P.M., Ward E., Thepapichaikul W., Tachampa K., Girolamo N.D., Brandão J. Evaluation Of Plasma Cardiac Troponin I In Asian Elephants (Elephas Maximus) Using Two Clinical Analyzers. Journal of Zoo and Wildlife Medicine Vol.53 No.4 (2023) , 654-660. 660. doi:10.1638/2021-0164 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/81468
Title
Evaluation Of Plasma Cardiac Troponin I In Asian Elephants (Elephas Maximus) Using Two Clinical Analyzers
Other Contributor(s)
Abstract
Cardiac troponin I (cTnI) is specific to myocardial tissue, highly conserved across taxa, and a reliable indicator of myocardial disease in human and veterinary medicine. Biomarkers, like cTnI, may be useful for cardiac evaluation of elephants because the application of other modalities is complicated by the size of the animal. The goal of this study was to establish observed ranges for plasma cTnI in Asian elephants (Elephas maximus) measured by two point-of-care analyzers. Blood was collected from captive juvenile (≤15 yr; n = 9), adult (16-50 yr; n = 42), and geriatric (>50 yr; n = 16) elephants. Following centrifugation, heparinized plasma was stored at 5°C prior to and in between analyses on iSTAT (Abbott Point of Care Inc, Princeton, NJ 08540, USA) and HUBI-QUANpro (Humiasis Co, Ltd, Anyang-si 14042, South Korea) analyzers. With the exception of two results, plasma concentrations of cTnI were below the limit of quantification (LOQ < 0.05 ng/ml) for the HUBI-QUANpro (n = 64), which prohibited comparison between the two analyzers. Observed ranges were determined for plasma cTnI concentrations reported by the iSTAT for the entire population sampled (n = 58; mean 0.011 ng/ml; SD ± 0.013 ng/ml; range 0.00-0.07 ng/ml; 95% CI 0.008-0.015 ng/ml; median 0.01 ng/ml) and with outliers excluded (n = 50; mean 0.007 ng/ml; SD ± 0.007 ng/ml; range 0.00-0.02 ng/ml; 95% CI 0.005-0.009 ng/ml; median 0.01 ng/ml). No significant differences were observed between age classes (P = 0.70) or sexes (P = 0.34). Higher cTnI concentrations were significantly correlated with increasing age (Pearson's r = 0.426; P = 0.002). Future studies are warranted to investigate the diagnostic potential of plasma cTnI in Asian elephants.