<sup>99m</sup>Tc /SPIO-loaded polymeric micelles as MRI and SPECT imaging, cancer-targeted nanoprobe for liver cancer detection
Issued Date
2023-01-01
Resource Type
ISSN
17732247
Scopus ID
2-s2.0-85143649644
Journal Title
Journal of Drug Delivery Science and Technology
Volume
79
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Drug Delivery Science and Technology Vol.79 (2023)
Suggested Citation
Assawapanumat W., Udomphon S., Kampaengtip A., Yaset S., Han X., Nittayacharn P., Nieman M.T., Chotipanich C., Sungkarat W., Sunintaboon P., Exner A.A., Nasongkla N. <sup>99m</sup>Tc /SPIO-loaded polymeric micelles as MRI and SPECT imaging, cancer-targeted nanoprobe for liver cancer detection. Journal of Drug Delivery Science and Technology Vol.79 (2023). doi:10.1016/j.jddst.2022.104060 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/82811
Title
<sup>99m</sup>Tc /SPIO-loaded polymeric micelles as MRI and SPECT imaging, cancer-targeted nanoprobe for liver cancer detection
Other Contributor(s)
Abstract
Magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT) imaging are combined to diagnose cancer with high physiological, anatomical, and metabolism sensitivity. For targeting hepatoma carcinoma, a drug delivery system is employed to carry drugs or chemicals to a specific area. Galactose is one of the sugar molecules which can be bound and increase uptake with asialoglycoprotein receptor (ASGP-R). In this study, superparamagnetic iron oxide nanoparticles (SPIONs), Galactose-functionalized poly(ethylene glycol)-block-poly(lactic acid) (Gal-PEG-b-PLA) and Diethylenetriaminepentaacetic acid-functionalized poly(ethylene glycol)-block-poly(lactic acid) (DTPA-PEG-b-PLA) were successfully synthesized and constructed to DTPA/Gal/SPIO micelles (targeting micelles) and DTPA/Met/SPIO micelles (non-targeting micelles). Technetium-99 m (99mTc) formed a complex with DTPA on the micelles as 99mTc/Gal/SPIO micelles by using stannous chloride and the pH was adjusted to acidic condition. The sizes of DTPA/Gal/SPIO micelles and DTPA/Met/SPIO micelles were lower than 100 nm with a spherical shape. In vitro study with HepG2-and L929-incubated micelles showed the intensity of blue staining of Prussian blue related to the amount of SPIO. 99mTc/Gal/SPIO micelles incubated with HepG2 exhibited signal intensity (SPECT signal) and relative R2* (MRI signal) higher than 99mTc/Met/SPIO micelles at the same time. According to these results, 99mTc-labeled Galactose-functionalized SPIO micelles have the potential for specific targeting to HepG2 and can aid in the diagnosis of cancer with MRI and SPECT dual imaging.