Development and Accuracy Evaluation of Lateral Flow Immunoassay for Rapid Diagnosis of Schistosomiasis Mekongi in Humans
Issued Date
2022-01-01
Resource Type
ISSN
15303667
eISSN
15577759
Scopus ID
2-s2.0-85123467372
Pubmed ID
34981973
Journal Title
Vector-Borne and Zoonotic Diseases
Volume
22
Issue
1
Start Page
48
End Page
54
Rights Holder(s)
SCOPUS
Bibliographic Citation
Vector-Borne and Zoonotic Diseases Vol.22 No.1 (2022) , 48-54
Suggested Citation
Rodpai R., Sadaow L., Sanpool O., Boonroumkaew P., Thanchomnang T., Laymanivong S., Janwan P., Limpanont Y., Chusongsang P., Ohmae H., Yamasaki H., Lv Z., Intapan P.M., Maleewong W. Development and Accuracy Evaluation of Lateral Flow Immunoassay for Rapid Diagnosis of Schistosomiasis Mekongi in Humans. Vector-Borne and Zoonotic Diseases Vol.22 No.1 (2022) , 48-54. 54. doi:10.1089/vbz.2021.0053 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87437
Title
Development and Accuracy Evaluation of Lateral Flow Immunoassay for Rapid Diagnosis of Schistosomiasis Mekongi in Humans
Author's Affiliation
Other Contributor(s)
Abstract
Schistosoma mekongi infection is endemic in countries along the Mekong River and certain of its tributaries in the lower Mekong basin, especially in Lao People's Democratic Republic and Cambodia. Diagnosis of schistosomiasis is crucial before treatment and epidemiological surveys before and/or after an intervention, such as a mass drug administration. A newly developed immunochromatographic test (ICT) for the diagnosis of schistosomiasis mekongi, based on antiparasite antibody detection in human sera, was evaluated. The schistosomiasis mekongi-ICT (Smk-ICT) strip was developed using somatic antigen from adult S. mekongi. In total, 209 serum samples were examined, including 14 from parasitologically proven schistosomiasis mekongi patients, 30 from schistosomiasis japonica patients, other parasitosis (n = 135), and healthy volunteers (n = 30) from areas not endemic for S. mekongi. Eleven schistosomiasis mekongi samples were positive according to the Smk-ICT, whereas all healthy control samples were negative. Cross-reactions with paragonimiasis heterotremus, sparganosis, trichinellosis, and taeniasis saginata samples were observed at 2.4% (4/165). The diagnostic sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 78.6% (95% confidence interval [CI] 49.2-95.3), 97.6% (95% CI 93.9-99.3), 73.3% (95% CI 44.9-92.2), 98.2% (95% CI 94.7-99.6), and 96.1% (95% CI 92.1-98.4), respectively. The Smk-ICT kit might be useful to assess the prevalence of disease before establishing transmission control and mass deworming campaigns in countries in the Mekong River subregion.