Two-stage acid-alkali pretreatment of vetiver grass to enhance the subsequent sugar release by cellulase digestion
Issued Date
2022-08-01
Resource Type
ISSN
09601481
eISSN
18790682
Scopus ID
2-s2.0-85133483116
Journal Title
Renewable Energy
Volume
195
Start Page
755
End Page
765
Rights Holder(s)
SCOPUS
Bibliographic Citation
Renewable Energy Vol.195 (2022) , 755-765
Suggested Citation
Huong V.T.T., Atjayutpokin T., Chinwatpaiboon P., Smith S.M., Boonyuen S., Luengnaruemitchai A. Two-stage acid-alkali pretreatment of vetiver grass to enhance the subsequent sugar release by cellulase digestion. Renewable Energy Vol.195 (2022) , 755-765. 765. doi:10.1016/j.renene.2022.06.069 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84552
Title
Two-stage acid-alkali pretreatment of vetiver grass to enhance the subsequent sugar release by cellulase digestion
Author's Affiliation
Other Contributor(s)
Abstract
Vetiver grass (VG; Chrysopogon zizanioides) can be used as a potential feedstock for biofuels and biochemical production due to its chemical composition, which is rich in cellulose, hemicellulose, and lignin. However, the complex and recalcitrant nature of a lignocellulosic biomass requires a pretreatment process to disrupt the lignin seal in the cell wall in order to increase the accessibility for enzymatic hydrolysis and to liberate the sugars contained within the cellulose. The sequential acid-base pretreatment of VG using dilute sulphuric acid (H2SO4) followed by sodium hydroxide (NaOH) was found to be optimal when using a 5% (w/v) VG loading in 0.5% (v/v) H2SO4 at 120 °C, 60 min in the first stage followed by 4% (w/v) NaOH at 120 °C, 60 min in the second stage. This gave the highest yield of total reducing sugars (32.6 g/L as 22.0 and 10.6 g/L of hexose and pentose, respectively) in the subsequent cellulase digestion, which was 10.3% and 46.1% higher than that obtained with the single-stage alkali (24.1 g/L) or acid (13.5 g/L) pretreatment, respectively.