Molecularly Imprinted Polymer-Amyloid Fibril-Based Electrochemical Biosensor for Ultrasensitive Detection of Tryptophan
Issued Date
2022-05-01
Resource Type
eISSN
20796374
Scopus ID
2-s2.0-85130201022
Pubmed ID
35624592
Journal Title
Biosensors
Volume
12
Issue
5
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biosensors Vol.12 No.5 (2022)
Suggested Citation
Alam I., Lertanantawong B., Sutthibutpong T., Punnakitikashem P., Asanithi P. Molecularly Imprinted Polymer-Amyloid Fibril-Based Electrochemical Biosensor for Ultrasensitive Detection of Tryptophan. Biosensors Vol.12 No.5 (2022). doi:10.3390/bios12050291 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83744
Title
Molecularly Imprinted Polymer-Amyloid Fibril-Based Electrochemical Biosensor for Ultrasensitive Detection of Tryptophan
Other Contributor(s)
Abstract
A tryptophan (Trp) sensor was investigated based on electrochemical impedance spectroscopy (EIS) of a molecularly imprinted polymer on a lysozyme amyloid fibril (MIP-AF). The MIP-AF was composed of aniline as a monomer chemically polymerized in the presence of a Trp template molecule onto the AF surface. After extracting the template molecule, the MIP-AF had cavities with a high affinity for the Trp molecules. The obtained MIP-AF demonstrated rapid Trp adsorption and substantial binding capacity (50 µM mg−1 ). Trp determination was studied using non-Faradaic EIS by drop drying the MIP-AF on the working electrode of a screen-printed electrode. The MIP-AF provided a large linear range (10 pM–80 µM), a low detection limit (8 pM), and high selectivity for Trp determination. Furthermore, the proposed method also indicates that the MIP-AF can be used to determine Trp in real samples such as milk and cancer cell media.