Development and Validation of a Rapid LC/MS-MS Method for Measuring Plasma Quetiapine and Norquetiapine in Psychiatric Patients
1
Issued Date
2025-10-01
Resource Type
ISSN
01252208
eISSN
24081981
Scopus ID
2-s2.0-105027894594
Journal Title
Journal of the Medical Association of Thailand
Volume
108
Start Page
S92
End Page
S100
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand Vol.108 (2025) , S92-S100
Suggested Citation
Mungkhunthod S., Prommas S., Puangpetch A., Paholpak P., Nakkam N., Sukasem C., Tassaneeyakul W., Vannaprasaht S. Development and Validation of a Rapid LC/MS-MS Method for Measuring Plasma Quetiapine and Norquetiapine in Psychiatric Patients. Journal of the Medical Association of Thailand Vol.108 (2025) , S92-S100. S100. doi:10.35755/jmedassocthai.2025.S02.S92-S100 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/114089
Title
Development and Validation of a Rapid LC/MS-MS Method for Measuring Plasma Quetiapine and Norquetiapine in Psychiatric Patients
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: Quetiapine (QTP) is an atypical antipsychotic drug that is widely used to treat psychiatric patients. N-desalkylquetiapine (NQTP) is the major and active metabolite. Objective: The present study aimed to develop and validate a method for measuring QTP and NQTP concentrations in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Materials and Methods: Plasma samples were processed using liquid-liquid extraction, and the analytes were separated on a C18 column. The analysis was conducted using a triple quadrupole tandem mass spectrometer with a mobile gradient phase consisting of 0.1% formic acid in 10 mM ammonium formate and 100% acetonitrile. The total run time was 6 min. Results: The calibration curves for QTP and NQTP were in a linear range of 2.0 to 600.0 ng/ml. The within-run and between-run precision for QTP was 10.29% and 11.16%, whereas those for NQTP were 8.21% and 12.87%, respectively. The within-run and between-run accuracy for QTP was <111.74%, <100.34%, <110.53%, and <104.11% for NQTP, respectively. The lower limit of detection for QTP and NQTP was 2.0 ng/ml. Conclusion: An LC-MS/MS method for measuring QTP and NQTP levels in human plasma was validated and successfully applied to measure these analytes in psychiatric patients.