A comparison of high-risk human papillomavirus DNA detection between urine and cervical sample testing in women with abnormal Pap smears
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Issued Date
2022-02-01
Resource Type
ISSN
13418076
eISSN
14470756
Scopus ID
2-s2.0-85118573836
Pubmed ID
34750932
Journal Title
Journal of Obstetrics and Gynaecology Research
Volume
48
Issue
2
Start Page
448
End Page
455
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Obstetrics and Gynaecology Research Vol.48 No.2 (2022) , 448-455
Suggested Citation
Punyashthira A. A comparison of high-risk human papillomavirus DNA detection between urine and cervical sample testing in women with abnormal Pap smears. Journal of Obstetrics and Gynaecology Research Vol.48 No.2 (2022) , 448-455. 455. doi:10.1111/jog.15095 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86185
Title
A comparison of high-risk human papillomavirus DNA detection between urine and cervical sample testing in women with abnormal Pap smears
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Aims: To compare the clinical performance of high-risk human papillomavirus (hrHPV) DNA detection between urine and cervical samples collected from the same patient for the detection of CIN2+ lesions (high-grade squamous intraepithelial lesions or cervical cancer lesions). The secondary objectives were to evaluate agreement among hrHPV genotypes and to compare patient satisfaction between urine and cervical sample collection. Methods: This prospective cross-sectional study enrolled 96 women with abnormal cervical cytology who attended the colposcopy clinic at Siriraj Hospital (Bangkok, Thailand) between July 2016 and January 2017. Self-collected random-voiding and first stream urine samples were collected into a universal sterile urine container and immediately mixing with preservative before the pelvic examination. Cervical tissue sampling was performed according to standard treatment guidelines. Both specimens were sent for extraction and detection of hrHPV by Anyplex II HPV high-risk testing. Study patients were surveyed to compare patient satisfaction between urine and cervical sample collection. Results: Carcinogenic hrHPV positive rate was 73% in urine samples and 81% in cervical samples. The sensitivity for HPV in the detection CIN2+ was high in both the urine and cervical groups at 86.2% and 94.8%, respectively. Agreement between the urine and cervical groups for HPV 16 or 18 detection was high, with kappa values of 0.86 for subtypes 16/18. Urine specimen collection had significantly higher satisfaction and acceptability than cervical specimen collection. Conclusion: Urine hrHPV testing by real-time polymerase chain reaction demonstrated high sensitivity and accuracy for the detection of CIN2+ lesions, with very good agreement when compared with cervical sample testing.