Role of lipid binding protein, Niemann pick type C-2, in enhancing shrimp sperm physiological function
Issued Date
2025-01-15
Resource Type
eISSN
24058440
Scopus ID
2-s2.0-85212815829
Journal Title
Heliyon
Volume
11
Issue
1
Rights Holder(s)
SCOPUS
Bibliographic Citation
Heliyon Vol.11 No.1 (2025)
Suggested Citation
Khongkha T., Rattanadechakul A., Surinlert P., Thongsum O., Boonkua S., Kongmanas K., Somrit M., Weerachatyanukul W., Asuvapongpatana S. Role of lipid binding protein, Niemann pick type C-2, in enhancing shrimp sperm physiological function. Heliyon Vol.11 No.1 (2025). doi:10.1016/j.heliyon.2024.e41341 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102838
Title
Role of lipid binding protein, Niemann pick type C-2, in enhancing shrimp sperm physiological function
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
Sperm activation occurring in both male and female reproductive tract involves a highly complex series of biomolecular dynamics, particularly on membrane lipids and proteins. In mammals, the universal anticipation in cholesterol (CHO) sequestration plays a role in mammalian sperm maturation/capacitation, subsequently enhancing sperm fertilizing ability. In shrimp, we have previously shown that the level of cholesterol (CHO) is significantly reduced in vas deferens sperm when compared with sperm in the testes, presumably due to the sequestering action of the lipid-binding protein, one of which is Niemann-Pick Type C-2 (NPC2). Here, we used recombinant MrNPC2 protein to treat testicular sperm (Tsp) and quantitatively compared the amount of CHO with non-treated or 2-OH-β-CD treated sperm. HPTLC of the extracted lipids from Macrobrachium rosenbergii sperm revealed the presence of major phospholipids and CHO. Tsp treated with 2-OH-β-CD or MrNPC2 showed lower CHO levels, which was comparable to that of the vas deferens sperm as verified by the Amplex Red assay. Finally, the enhanced levels of protein tyrosine phosphorylation and ionophore-induced AR levels in the MrNPC2 treated Tsp significantly increased in a concentration-dependent manner, similar to that of the Vsp sperm. Altogether, our results indicated the importance of MrNPC2 as CHO moderator in sperm membrane, leading to an enhancement in sperm fertilizing ability.