Salivary Oxidative Stress Biomarkers in Thai Adolescents and Young Adults with Type 1 Diabetes Mellitus: A Cross-Sectional Study
Issued Date
2023-01-01
Resource Type
ISSN
22310762
eISSN
22501002
Scopus ID
2-s2.0-85171572830
Journal Title
Journal of International Society of Preventive and Community Dentistry
Volume
13
Issue
4
Start Page
333
End Page
341
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of International Society of Preventive and Community Dentistry Vol.13 No.4 (2023) , 333-341
Suggested Citation
Aroonrangsee T., Chairatnathrongporn R., Surarit R., Tansriratanawong K., Santiprabhob J., Boriboonhirunsarn C., Promsudthi A. Salivary Oxidative Stress Biomarkers in Thai Adolescents and Young Adults with Type 1 Diabetes Mellitus: A Cross-Sectional Study. Journal of International Society of Preventive and Community Dentistry Vol.13 No.4 (2023) , 333-341. 341. doi:10.4103/jispcd.JISPCD_38_23 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/90216
Title
Salivary Oxidative Stress Biomarkers in Thai Adolescents and Young Adults with Type 1 Diabetes Mellitus: A Cross-Sectional Study
Author's Affiliation
Other Contributor(s)
Abstract
Aims and Objectives: The primary objectives of this study were to compare salivary oxidative stress (OS) biomarker levels in patients with type 1 diabetes mellitus (T1DM) and without T1DM (non-T1DM) and evaluate the relationships between diabetes, periodontal status, and OS biomarker levels. Materials and Methods: Twenty patients with T1DM and 20 age-matched patients without T1DM were enrolled. All participants were 15-23 years of age and had permanent dentition. Unstimulated whole saliva was collected in a sterile test tube before examination of clinical periodontal parameters, including bleeding on probing (BOP). Salivary levels of OS biomarkers - malondialdehyde, protein carbonyl, total oxidant status (TOS), and total antioxidant capacity - were determined using oxidative and antioxidative assays followed by spectrophotometric measurement at 375-532 nm. The relationships between diabetes, periodontal status, and OS biomarkers were analyzed using multiple linear regression. Results: TOS was significantly lower in the T1DM group compared with the non-T1DM group (5.06 ± 0.39 vs. 6.44 ± 0.51 μmol H 2 O 2 Eq/l, P = 0.035). After adjusting for confounding factors (age, gender, BMI, clinical periodontal parameters, BOP, or diabetes status accordingly), the multiple linear regression showed that T1DM was significantly associated with a reduction of TOS level (P = 0.008). The BOP > 30% group showed a significant correlation with increased TOS levels compared with the BOP ≤ 30% group (P = 0.002). No relationship was found between OS biomarkers and HbA1c levels. Conclusion: Salivary TOS levels were related to both diabetes status and the extent of gingival inflammation. Further studies to elucidate the role of OS in relation of periodontal disease and T1DM are required.