Effects of pectin on blood toxicity of lead and mutagenicity of 4-nitro-o-phenylenediamine and sodium azide
2
Issued Date
2024
Copyright Date
1992
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xiii, 108 leaves : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Toxicology))--Mahidol University, 1992
Suggested Citation
Arnurai Ruksakun Effects of pectin on blood toxicity of lead and mutagenicity of 4-nitro-o-phenylenediamine and sodium azide. Thesis (M.Sc. (Toxicology))--Mahidol University, 1992. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/99836
Title
Effects of pectin on blood toxicity of lead and mutagenicity of 4-nitro-o-phenylenediamine and sodium azide
Alternative Title(s)
ผลของเพคตินต่อพิษจากสารตะกั่วทางโลหิตวิทยาและการก่อกลายพันธุ์ของ 4- ไนโตร-โอ-เฟนิลลีนไดอะมีนและโซเดียมเอไซด์
Author(s)
Abstract
This study was conducted to investigate the effects of pectin on mutagens in Ames test and lead intoxication. This experiment divided into 2 studies. In study 1, the effects of pectin on mutagens in Ames test. Pectin, 2.5, 5.0 and 10.0 mg were incubated for 30 minutes with aqueous solution of 4-nitro-o-phenylenediamine (NPD) 50.0 µg, 100.0 µg /ml or sodium azide 5.0 µg, 10.0 µg/ml, centrifuged. The Ames assay were performed with supernatant and Salmonella typhimurium TA 98 or TA 100 respectively without metabolic activation to determine any changes in mutagenicity of NPD and sodium azide. Mutagenic activities of NPD or sodium azide were decreased by pectin 6.3% to 15.0% and 10.7% to 26.9% respectively. The complex mechanism may be involved in binding of mutagen and/or adsorption with pectin, it is limited to this study. In study 2, Influence of a pectin diet to reduce toxicity in rats induced by lead acetate. Rats (65-75 g) were fed for 6 weeks ad libitum either basal diet or basal diet containing 15% pectin and then orally administered lead acetate weekly. During the last day of the experiment, the urine was collected and quantitative measurement of urinary lead and δ-aminolevulinic acid (ALA) were carried out. The lead level, δ-aminolevulinic acid dehydratase (ALA-D), free erythrocyte protoporphyrin (FEP), hematological and biochemical parameters in blood were determined. The blood lead level was not different between the rats receiving 15% pectin and basal diets of controls and rats treated with lead acetate 1,000, 2,000 mg/kg BW/wk (5.0 ± 0.56 VS 4.38 ± 1.46, 49.38 ± 5.69 VS 55.62 ± 4.20, 90.62 ± 11.46 VS 96.62 ± 9.48 respectively). The level of ALA-D and FEP in blood and lead level, ALA in urine were not different in both groups. The red blood cells parameters (RBC count, Hb, Hct, MCV, MCH, MCHC, RDW, HDW) were also not different for rats receiving 15% pectin and basal diets. The white blood cell counts and various white blood cells were uninfluenced by either lead exposure or the control group. The biochemical parameters, liver and kidney function, were also not different of rats fed both diets. The rats fed 15% pectin had less weight gain in all groups including control rats (no lead acetate) (4.18 ± 0.17 VS 5.72 ± 0-.21, 4.09 ± 0.19 VS 4.89 ± 0.12, 3.77±0.27 VS 4.75±0.13, p<0.001, p<0.01 respectively). The result indicated that pectin may not alter lead absorption and excretion of lead in urine, nor did it reduce lead intoxication. However, the rats fed 15% pectin can maintain systemic lead intoxication despite less weight gain.
Description
Toxicology (Mahidol University 1992)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Toxicology
Degree Grantor(s)
Mahidol University