An irreversible paper-based profluorescent nitroxide probe for the selective detection of ascorbic acid
8
item.page.dc.date.issued
2023-09-18
item.page.dc.type
item.page.dc.identifier.eissn
20462069
item.page.dc.identifier.doi
item.page.dc.identifier.scopus
2-s2.0-85172423113
item.page.oaire.citation.title
RSC Advances
item.page.oaire.citation.volume
13
item.page.oaire.citation.issue
39
item.page.oaire.citation.startPage
27663
item.page.oaire.citation.endPage
27671
item.page.dc.rights.holder
SCOPUS
item.page.dc.identifier.citation
RSC Advances Vol.13 No.39 (2023) , 27663-27671
punsarn.item.page.citation
Decha N., Sirirak J., Sooksawat D., Phonchai A., Pornsuwan S., Tansakul C. An irreversible paper-based profluorescent nitroxide probe for the selective detection of ascorbic acid. RSC Advances Vol.13 No.39 (2023) , 27663-27671. 27671. doi:10.1039/d3ra04681h Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/90346
item.page.dc.title
An irreversible paper-based profluorescent nitroxide probe for the selective detection of ascorbic acid
item.page.dc.contributor.author
item.page.oairecerif.author.affiliation
item.page.dc.contributor.other
item.page.dc.description.abstract
Ascorbic acid (AA) or vitamin C plays multiple crucial roles, particularly as an antioxidant. This essentially biologically active molecule was selectively detected over other reductants by the synthesized profluorescent nitroxide probe ProN6via a switch-on method. After either a hydrogen atom or single electron transfer from AA to nitroxide, the resulting diamagnetic hydroxylamine was rapidly cyclized to form a fluorescent O-acylalkoxyamine. This cyclization prevented the reoxidation of the corresponding hydroxylamine to the nitroxide, leading to a high precision of detection. A kinetic fluorescence study indicated that ProN6 exhibited higher reactivity than ProN7. Density functional theory (DFT) calculations indicated that the Gibbs free energy of the AA-induced cascade reductive lactonization of ProN6 was lower than that of ProN5 and ProN7. The designed probe achieved the sensitive and specific detection of AA with detection limits of 77.9 nM and 195.9 μM in solution and on paper, respectively. The utilization of the probe as a paper-based fluorescent sensor demonstrated the good accuracy of the quantitative analysis of AA in commercial supplements.