Biochemical characterization and inhibitor potential of African swine fever virus thymidine kinase
Issued Date
2025-03-01
Resource Type
ISSN
01418130
eISSN
18790003
Scopus ID
2-s2.0-85214296625
Pubmed ID
39743116
Journal Title
International Journal of Biological Macromolecules
Volume
293
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Biological Macromolecules Vol.293 (2025)
Suggested Citation
Rasri N., Kornyanee C., Srisanga K., Nutho B., Chanarat S., Kuhaudomlarp S., Tinikul R., Pakotiprapha D. Biochemical characterization and inhibitor potential of African swine fever virus thymidine kinase. International Journal of Biological Macromolecules Vol.293 (2025). doi:10.1016/j.ijbiomac.2024.139391 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102967
Title
Biochemical characterization and inhibitor potential of African swine fever virus thymidine kinase
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Abstract
African Swine Fever (ASF) is a highly contagious disease affecting both domestic pigs and wild boars. In domestic pigs, ASF is a rapidly-progressing disease with a mortality rate reaching 100 %, causing tremendous economic loss in affected areas. ASFV is caused by African Swine Fever Virus (ASFV), which is a large, enveloped double-stranded DNA virus belonging to the Asfarviridae family. ASFV has a remarkably large genome size that encodes more than 150 open reading frames. Among the virally encoded enzymes, thymidine kinase (ASFV-TK) has been shown to be critical for the efficient replication and virulence of ASFV. Here, we report the bioinformatics analysis and biochemical characterization of ASFV-TK. Amino acid sequence analysis revealed that ASFV-TK can be classified as a type II thymidine kinase. Kinetics characterization revealed a maximum velocity (Vmax) and Michaelis constants (Km) that are within the same range as previously characterized type II enzymes. ASFV-TK is competitively inhibited by the feedback inhibitor thymidine 5′-triphosphate and can use 3′-azido-3′-deoxythymidine (AZT) as a substrate with kinetics parameters comparable to those obtained with natural substrates, suggesting that nucleosides and nucleotide analogs could be explored as anti-ASFV agents.