Specific Interaction of DARPin with HIV-1 CANTD Disturbs the Distribution of Gag, RNA Packaging, and Tetraspanin Remodelling in the Membrane
2
Issued Date
2022-04-01
Resource Type
eISSN
19994915
Scopus ID
2-s2.0-85128801278
Pubmed ID
35458554
Journal Title
Viruses
Volume
14
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
Viruses Vol.14 No.4 (2022)
Suggested Citation
Moonmuang S., Maniratanachote R., Chetprayoon P., Sornsuwan K., Thongkum W., Chupradit K., Tayapiwatana C. Specific Interaction of DARPin with HIV-1 CANTD Disturbs the Distribution of Gag, RNA Packaging, and Tetraspanin Remodelling in the Membrane. Viruses Vol.14 No.4 (2022). doi:10.3390/v14040824 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/84999
Title
Specific Interaction of DARPin with HIV-1 CANTD Disturbs the Distribution of Gag, RNA Packaging, and Tetraspanin Remodelling in the Membrane
Other Contributor(s)
Abstract
A designed repeat scaffold protein (AnkGAG1D4) recognizing the human immunodefi-ciency virus-1 (HIV-1) capsid (CA) was formerly established with antiviral assembly. Here, we in-vestigated the molecular mechanism of AnkGAG 1D4 function during the late stages of the HIV-1 replication cycle. By applying stimulated emission-depletion (STED) microscopy, Gag polymerisation was interrupted at the plasma membrane. Disturbance of Gag polymerisation triggered Gag accumulation inside producer cells and trapping of the CD81 tetraspanin on the plasma membrane. Moreover, reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) experiments were performed to validate the packaging efficiency of RNAs. Our results advocated that AnkGAG 1D4 interfered with the Gag precursor protein from selecting HIV-1 and cellular RNAs for encapsidation into viral particles. These findings convey additional information on the antiviral activity of AnkGAG1D4 at late stages of the HIV-1 life cycle, which is potential for an alternative anti-HIV molecule.