Enhancing Erythropoiesis by a Phytoestrogen Diarylheptanoid from Curcuma comosa
2
Issued Date
2022-06-01
Resource Type
eISSN
22279059
Scopus ID
2-s2.0-85132768202
Journal Title
Biomedicines
Volume
10
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biomedicines Vol.10 No.6 (2022)
Suggested Citation
Bhukhai K., Fouquet G., Rittavee Y., Tanhuad N., Lakmuang C., Borwornpinyo S., Anurathapan U., Suksamrarn A., Piyachaturawat P., Chairoungdua A., Hermine O., Hongeng S. Enhancing Erythropoiesis by a Phytoestrogen Diarylheptanoid from Curcuma comosa. Biomedicines Vol.10 No.6 (2022). doi:10.3390/biomedicines10061427 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83706
Title
Enhancing Erythropoiesis by a Phytoestrogen Diarylheptanoid from Curcuma comosa
Other Contributor(s)
Abstract
Erythropoietin (Epo) is widely used for the treatment of anemia; however, non-hematopoietic effects and cancer risk limit its clinical applications. Therefore, alternative molecules to improve erythropoiesis in anemia patients are urgently needed. Here, we investigated the potential effects of a phytoestrogen diarylheptanoid (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol, (ASPP 049) isolated from Curcuma comosa on promoting erythropoiesis. Treatment with C. comosa extract improved anemia symptoms demonstrated by increasing red blood cell numbers, hematocrit, and hemoglobin content in anemic mice. In addition, ASPP 049, the major compound isolated from C. comosa, enhanced the suboptimal Epo dosages to improve erythroid cell differentiation from hematopoietic stem cells, which was inhibited by the estrogen receptor (ER) antagonist, ICI 182,780. Moreover, the ASPP 049-activated Epo-Epo receptor (EpoR) complex subsequently induced phosphorylation of EpoR-mediated erythropoiesis pathways: STAT5, MAPK/ERK, and PI3K/AKT in Epo-sensitive UT-7 cells. Taken together, these results suggest that C. comosa extract and ASPP 049 increased erythropoiesis through ER-and EpoR-mediated signaling cascades. Our findings provide insight into the specific interaction between a phytoestrogen diarylheptanoid and Epo-EpoR in a hematopoietic system for the potential treatment of anemia.