In vitro drug susceptibility using a parasite-rescue and transformation assay of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis amastigotes against antileishmanial drugs

dc.contributor.authorKimcharoensuk S.
dc.contributor.authorLeelayoova S.
dc.contributor.authorMungthin M.
dc.contributor.authorNawattanapaibool N.
dc.contributor.authorTan-ariya P.
dc.contributor.authorSiripattanapipong S.
dc.contributor.otherMahidol University
dc.date.accessioned2023-12-09T18:01:52Z
dc.date.available2023-12-09T18:01:52Z
dc.date.issued2024-01-01
dc.description.abstractLeishmaniasis is an emerging infectious disease in Thailand, with Leishmania martiniquensis and Leishmania orientalis identified as the primary causative agents among immunocompetent and immunocompromised individuals. Variations in drug susceptibility among different Leishmania species have been reported in different regions. Therefore, drug susceptibility assays are essential to assess the effectiveness of antileishmanial drugs used or potentially used in the affected areas. This study investigated the in vitro drug sensitivity of L. martiniquensis and L. orientalis, along with two reference species causing VL, namely L. donovani and L. infantum, against six antileishmanial drugs. Using a parasite-rescue and transformation assay, the results demonstrated that the IC50 values of amphotericin B (AmB), miltefosine (MIL), and sodium stibogluconate (Sb(III)) against all Leishmania species tested were within the sensitive range of each drug. On the contrary, the IC50 values of artemisinin (ART) and dihydroartemisinin (DHA), drugs primarily used for malaria treatment, were outside the sensitive range of the Leishmania species tested except L. infantum. This in vitro study highlights that AmB could effectively exhibit good sensitivity against the intracellular amastigotes of L. martiniquensis and L. orientalis. Also, MIL and Sb(III) could be considered alternative drugs for antileishmanial treatment in Thailand.
dc.identifier.citationActa Tropica Vol.249 (2024)
dc.identifier.doi10.1016/j.actatropica.2023.107081
dc.identifier.eissn18736254
dc.identifier.issn0001706X
dc.identifier.pmid37993039
dc.identifier.scopus2-s2.0-85178131575
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/91355
dc.rights.holderSCOPUS
dc.subjectImmunology and Microbiology
dc.titleIn vitro drug susceptibility using a parasite-rescue and transformation assay of Leishmania (Mundinia) martiniquensis and Leishmania (Mundinia) orientalis amastigotes against antileishmanial drugs
dc.typeArticle
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85178131575&origin=inward
oaire.citation.titleActa Tropica
oaire.citation.volume249
oairecerif.author.affiliationMahidol University
oairecerif.author.affiliationPhramongkutklao College of Medicine

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